Egido J M, Viñuelas J
Department of Microbiology and Inmunology, Santiago University, Spain.
Rev Soc Bras Med Trop. 1997 Nov-Dec;30(6):441-6. doi: 10.1590/s0037-86821997000600001.
We report a rapid method for the flow cytometric quantitation of phagocytosis in heparinized complete peripheral blood (HCPB), using commercially available phycoerythrin-conjugated latex particles of 1 micron diameter. The method is faster and shows greater reproducibility than Bjerknes' (1984) standard technique using propidium iodide-stained Candida albicans, conventionally applied to the leukocytic layer of peripheral blood but here modified for HCPB. We also report a modification of Bjerknes' Intracellular Killing Test to allow its application to HCPB.
我们报告了一种快速方法，用于对肝素化全外周血（HCPB）中的吞噬作用进行流式细胞术定量分析，该方法使用市售的直径为1微米的藻红蛋白缀合乳胶颗粒。与Bjerknes（1984年）使用碘化丙啶染色的白色念珠菌的标准技术相比，该方法更快且重现性更高，传统上该标准技术应用于外周血的白细胞层，但在此处针对HCPB进行了改进。我们还报告了对Bjerknes细胞内杀伤试验的改进，以使其能够应用于HCPB。
