通过间期和中期荧光原位杂交技术检测慢性淋巴细胞白血病中的12号染色体三体和着丝粒改变。

Detection of trisomy 12 and centromeric alterations in CLL by interphase- and metaphase-FISH.

作者信息

Acar H, Connor M J

机构信息

Department of Medical Genetics, University of Selçuk, Konya, Turkey.

出版信息

Cancer Genet Cytogenet. 1998 Jan 15;100(2):148-51. doi: 10.1016/s0165-4608(97)00024-1.

DOI:10.1016/s0165-4608(97)00024-1

PMID:9428359

Abstract

We have studied trisomy 12 in chronic lymphocytic leukemia (CLL) by fluorescence in situ hybridization (FISH) with an alpha-satellite centromeric probe for chromosome 12 on both dividing and non-dividing cells. Trisomy for chromosome 12 was demonstrated in four of these patients (15.3%) using FISH on interphase cells. The percentage of trisomic cells ranged from 10% to 65% of nuclei. The hybridization signals in the trisomic and disomic nuclei were of a broadly similar size and nature. Interestingly, three of the remaining CLL patients, who exhibited disomy for chromosome 12, showed a marked difference in size of the hybridization signals in interphase nuclei. This was also demonstrated in metaphase spreads. In addition, metaphase FISH studies revealed a supernumerary marker chromosome in three out of 26 patients with CLL.

摘要

我们通过荧光原位杂交（FISH）技术，使用针对12号染色体的α卫星着丝粒探针，对处于分裂期和非分裂期的细胞进行研究，以检测慢性淋巴细胞白血病（CLL）中的12号染色体三体情况。在这些患者中，有4例（15.3%）通过间期细胞FISH检测证实存在12号染色体三体。三体细胞的百分比在细胞核的10%至65%之间。三体细胞核和二体细胞核中的杂交信号在大小和性质上大致相似。有趣的是，其余表现为12号染色体二体的3例CLL患者，其间期细胞核中杂交信号的大小存在显著差异。这在中期染色体铺展中也得到了证实。此外，中期FISH研究显示，26例CLL患者中有3例存在一条额外的标记染色体。