Chen R, Amoui M, Zhang Z, Mardon G
Department of Pathology, Baylor College of Medicine, Houston, Texas 77030, USA.
Cell. 1997 Dec 26;91(7):893-903. doi: 10.1016/s0092-8674(00)80481-x.
The eyeless, dachshund, and eyes absent genes encode conserved, nuclear proteins that are essential for eye development in Drosophila. Misexpression of eyeless or dachshund is also sufficient to induce the formation of ectopic compound eyes. Here we show that the dachshund and eyes absent genes act synergistically to induce ectopic retinal development and positively regulate the expression of each other. Moreover, we show that the Dachshund and Eyes Absent proteins can physically interact through conserved domains, suggesting a molecular basis for the genetic synergy observed and that a similar complex may function in mammals. We propose that a conserved regulatory network, rather than a linear hierarchy, controls retinal specification and involves multiple protein complexes that function during distinct steps of eye development.
无眼基因、腊肠犬基因和无眼相关基因编码保守的核蛋白，这些蛋白对果蝇眼睛发育至关重要。无眼基因或腊肠犬基因的错误表达也足以诱导异位复眼的形成。在此我们表明，腊肠犬基因和无眼相关基因协同作用以诱导异位视网膜发育，并相互正向调节彼此的表达。此外，我们表明腊肠犬蛋白和无眼相关蛋白可通过保守结构域进行物理相互作用，这为观察到的遗传协同作用提供了分子基础，并且类似的复合物可能在哺乳动物中发挥作用。我们提出，一个保守的调控网络而非线性层级结构控制视网膜特化，并且涉及在眼睛发育不同阶段发挥作用的多个蛋白复合物。
