Pallaghy P K, Alewood D, Alewood P F, Norton R S
Biomolecular Research Institute, Parkville, Vic., Australia.
FEBS Lett. 1997 Dec 15;419(2-3):191-6. doi: 10.1016/s0014-5793(97)01452-x.
The solution structure of robustoxin, the lethal neurotoxin from the Sydney funnel-web spider Atrax robustus, has been determined from 2D 1H NMR data. Robustoxin is a polypeptide of 42 residues cross-linked by four disulphide bonds, the connectivities of which were determined from NMR data and trial structure calculations to be 1-15, 8-20, 14-31 and 16-42 (a 1-4/2-6/3-7/5-8 pattern). The structure consists of a small three-stranded, anti-parallel beta-sheet and a series of interlocking gamma-turns at the C-terminus. It also contains a cystine knot, thus placing it in the inhibitor cystine knot motif family of structures, which includes the omega-conotoxins and a number of plant and animal toxins and protease inhibitors. Robustoxin contains three distinct charged patches on its surface, and an extended loop that includes several aromatic and non-polar residues. Both of these structural features may play a role in its binding to the voltage-gated sodium channel.
已根据二维¹H NMR数据确定了悉尼漏斗网蜘蛛（Atrax robustus）的致死性神经毒素——强劲毒素的溶液结构。强劲毒素是一种由42个残基组成的多肽，通过四个二硫键交联，其连接方式通过NMR数据和试验性结构计算确定为1-15、8-20、14-31和16-42（1-4/2-6/3-7/5-8模式）。该结构由一个小的三链反平行β-折叠和C端一系列互锁的γ-转角组成。它还包含一个胱氨酸结，因此将其归入抑制剂胱氨酸结基序结构家族，该家族包括ω-芋螺毒素以及许多动植物毒素和蛋白酶抑制剂。强劲毒素在其表面有三个不同的带电区域，以及一个包含多个芳香族和非极性残基的延伸环。这两个结构特征可能都在其与电压门控钠通道的结合中发挥作用。
