Collins S P, Comis A, Tyler M I, Marshall M, Howden M E
School of Biological and Chemical Sciences, Deakin University, Geelong, Vic., Australia.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1995 Jan;110(1):89-93. doi: 10.1016/0742-8413(95)93865-t.
Robustoxin is the lethal polypeptide toxin in Atrax robustus venom. A monoclonal antibody was produced using synthetic, unfolded robustoxin conjugated to keyhole limpet haemocyanin as the immunogen. This monoclonal antibody did not protect newborn mice against challenge with the crude venom of the male Sydney funnel-web spider, but did slightly prolong their survival time. Western blotted crude venom of the male Sydney funnel-web spider showed two monoclonal antibody binding bands. One band at low M(r) corresponded to robustoxin (M(r) 4854), while the other higher M(r) band (approximately 37,000) may be due to a pre-robustoxin molecule.
强劲毒素是粗短漏斗蛛毒液中的致死性多肽毒素。以与钥孔戚血蓝蛋白偶联的合成、未折叠的强劲毒素作为免疫原制备了单克隆抗体。这种单克隆抗体不能保护新生小鼠免受雄性悉尼漏斗网蜘蛛粗毒液的攻击，但确实略微延长了它们的存活时间。雄性悉尼漏斗网蜘蛛粗毒液的蛋白质印迹显示有两条单克隆抗体结合带。低分子量（M(r)）处的一条带对应于强劲毒素（M(r) 4854），而另一条较高分子量（约37,000）的带可能归因于前强劲毒素分子。
