Voronov I, Santerre J P, Hinek A, Callahan J W, Sandhu J, Boynton E L
Department of Chemical Engineering, University of Toronto, Canada.
J Biomed Mater Res. 1998 Jan;39(1):40-51. doi: 10.1002/(sici)1097-4636(199801)39:1<40::aid-jbm6>3.0.co;2-i.
In this study, an in vitro model has been developed to examine the interactions of macrophages with ultrahigh molecular-weight polyethylene (UHMWPE) and high-density polyethylene (HDPE) particles. Polyethylene particles are the major constituent of the material debris formed as a result of orthopedic implant wear. However, the study of polyethylene particle interactions with cells has been limited. UHMWPE (18-20 microns) and HDPE (4-10 microns) were suspended in soluble collagen type I and subsequently solidified on glass coverslips. The particle chemistry was characterized by Fourier transform infra-red spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS). Mouse cell line macrophages (IC-21) were established on the collagen-particle substrata and maintained for up to 24 h. The response of the cells to the particles was examined by light and transmission electron microscopy (LM and TEM), as well as by scanning electron microscopy (SEM), and compared to cells on control collagen surfaces without particles. Histological analysis of the samples revealed that the macrophages surrounded larger particles (18-20 microns) and the cells appeared to be attached to the surface of the particles, and the smaller particles (4-10 microns) had been phagocytosed within 2 h. Inflammatory cytokines (TNF-alpha, IL-1 alpha, IL-1 beta, and IL-6), lysosomal enzymes (beta-galactosidase and hexosaminidase), and prostaglandin E2 were released into the medium, and IL-1 alpha, IL-1 beta, PGE2, beta-galactosidase, and hexosaminidase levels were significantly increased over collagen control values. The results demonstrate active phagochemotaxis by macrophages for wear particulates and validate this model as a means of studying the specific in vitro interactions of polyethylene with cells.
在本研究中，已建立一种体外模型来检测巨噬细胞与超高分子量聚乙烯（UHMWPE）和高密度聚乙烯（HDPE）颗粒之间的相互作用。聚乙烯颗粒是骨科植入物磨损产生的材料碎片的主要成分。然而，聚乙烯颗粒与细胞相互作用的研究一直很有限。将UHMWPE（18 - 20微米）和HDPE（4 - 10微米）悬浮于I型可溶性胶原蛋白中，随后在玻璃盖玻片上固化。通过傅里叶变换红外光谱（FT - IR）和X射线光电子能谱（XPS）对颗粒化学性质进行表征。将小鼠细胞系巨噬细胞（IC - 21）接种在胶原蛋白 - 颗粒基质上，并维持长达24小时。通过光学显微镜和透射电子显微镜（LM和TEM）以及扫描电子显微镜（SEM）检测细胞对颗粒的反应，并与无颗粒的对照胶原蛋白表面上的细胞进行比较。样品的组织学分析显示，巨噬细胞围绕较大颗粒（18 - 20微米），细胞似乎附着在颗粒表面，较小颗粒（4 - 10微米）在2小时内被吞噬。炎性细胞因子（TNF -α、IL - 1α、IL - 1β和IL - 6）、溶酶体酶（β - 半乳糖苷酶和己糖胺酶）以及前列腺素E2释放到培养基中，并且IL - 1α、IL - 1β、PGE2、β - 半乳糖苷酶和己糖胺酶水平相对于胶原蛋白对照值显著升高。结果表明巨噬细胞对磨损颗粒具有活跃的吞噬趋化作用，并验证了该模型作为研究聚乙烯与细胞特定体外相互作用的一种手段。
