Petit Institute for Bioengineering and Bioscience, Georgia Institute of Technology, Atlanta, Georgia 30332-0363, USA.
J Biomed Mater Res A. 2010 Jul;94(1):252-8. doi: 10.1002/jbm.a.32669.
Inflammatory responses to implanted biomedical devices elicit a foreign body fibrotic reaction that limits device integration and performance in various biomedical applications. We examined chronic inflammatory responses to microgel conformal coatings consisting of thin films of poly(N-isopropylacrylamide) hydrogel microparticles cross-linked with poly(ethylene glycol) diacrylate deposited on poly(ethylene terephthalate) (PET). Unmodified and microgel-coated PET disks were implanted subcutaneously in rats for 4 weeks and explants were analyzed by histology and immunohistochemistry. Microgel coatings reduced chronic inflammation and resulted in a more mature/organized fibrous capsule. Microgel-coated samples exhibited 22% thinner fibrous capsules that contained 40% fewer cells compared to unmodified PET disks. Furthermore, microgel-coated samples contained significantly higher levels of macrophages (80%) than unmodified PET controls. These results demonstrate that microgel coatings reduce chronic inflammation to implanted biomaterials. (c) 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010.
植入式生物医学设备引发的炎症反应会引起异物纤维性反应，从而限制各种生物医学应用中设备的整合和性能。我们研究了聚（N-异丙基丙烯酰胺）水凝胶微颗粒薄膜交联的聚（乙二醇）二丙烯酸酯微凝胶共形涂层对慢性炎症反应的影响，这些微颗粒薄膜沉积在聚对苯二甲酸乙二醇酯（PET）上。未修饰和微凝胶涂层的 PET 圆盘分别皮下植入大鼠体内 4 周，通过组织学和免疫组织化学分析植入物。微凝胶涂层减少了慢性炎症反应，并导致更成熟/有序的纤维囊。与未修饰的 PET 圆盘相比，微凝胶涂层的样品具有 22%更薄的纤维囊，其中的细胞数量减少了 40%。此外，微凝胶涂层的样品中巨噬细胞（80%）的含量明显高于未修饰的 PET 对照。这些结果表明，微凝胶涂层可减少植入生物材料的慢性炎症反应。（c）2010 年 Wiley 期刊，Inc. J 生物医学材料研究，2010 年。
