Jardine J E, Vogel S W, van Kleef M, van der Lugt J J
Department of Pathology, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, South Africa.
Onderstepoort J Vet Res. 1995 Dec;62(4):277-80.
An immunohistochemical staining technique in which a monospecific serum was used against the major antigenic protein -1 (MAP-1) of Cowdria ruminantium, was evaluated for the detection of C. ruminantium in formalin-fixed tissues of experimentally infected mice and field cases of heartwater in sheep, cattle and goats. Mice were infected with the mouse-pathogenic stocks: Mara, Kwanyanga, Welgevonden, Nonile, Vosloo, Kümm, Mali and Omatjenne. In all these cases and in the naturally infected cattle, sheep and goats, Cowdria colonies were identified as clearly-defined, brown-staining rickettsial colonies within the cytoplasm of endothelial cells. No positive staining was observed in the control group. This technique was shown to be reliable for detecting infection with C. ruminantium in the formalin-fixed tissues of mice and domestic ruminants.
采用一种免疫组织化学染色技术，该技术使用针对反刍兽考德里氏体主要抗原蛋白-1（MAP-1）的单特异性血清，用于检测实验感染小鼠的福尔马林固定组织以及绵羊、牛和山羊心水病现场病例中的反刍兽考德里氏体。小鼠感染了对小鼠致病的毒株：马拉、夸延加、韦尔格冯登、诺尼尔、沃斯洛、库姆、马里和奥马延内。在所有这些病例以及自然感染的牛、绵羊和山羊中，考德里氏体菌落被鉴定为内皮细胞胞质内清晰界定的棕色染色立克次体菌落。对照组未观察到阳性染色。结果表明，该技术对于检测小鼠和家养反刍兽福尔马林固定组织中的反刍兽考德里氏体感染是可靠的。
