Ortega Cerrilla M E, Reyes Ortigoza A L, Mendoza Martínez G
Arch Latinoam Nutr. 1996 Dec;46(4):325-8.
Earthworms (Eisenia fetida and Lumbricus rubellus) were ensiled with ground sorghum and molasses in the following proportions: 1) 60% earthworms, 40% sorghum; 2) 60% earthworms, 40% sorghum, adjusting pH to 4.0 with HCl; 3) 60% earthworms, 20% sorghum, 20% molasses; 4) 60% earthworms, 20% sorghum, 20% molasses, adjusting pH to 4.0 with HCl. These mixtures were allowed to ferment for 15 days at 18 degrees C. pH, proximate chemical analyses, digestible protein, true protein, ammonia nitrogen, acetic, propionic and butyric acid, lactic acid and gross energy were analyzed in the ensiled mixtures. Data were analyzed by ANOVA and orthogonal contrasts. No differences (P > 0.0001) were found in the percentage of moisture, ether extract, crude fiber and crude protein (52.22, 2.96, 1.15, 22.00, 51.76, 3.48, 1.28, 20.17; 53.89, 3.23, 0.95, 20.63; 54.87, 2.99, 1.03, 21.14, for treatments 1, 2, 3 and 4, respectively). Neither there was any difference (P > 0.0001) for true protein and gross energy (7.57, 4.37; 6.92, 4.41; 5.45, 4.37; 6.38, 4.30, for treatments 1, 2, 3 and 4, respectively). Ash content (P < 0.0001) and nitrogen free extract (p < 0.02) were different between treatments with sorghum and treatments with sorghum and molasses (3.80, 70.09; 3.60, 71.47; 6.08, 69.11; 6.63, 68.21, for treatments 1, 2, 3 and 4, respectively). Digestible protein was also different (P < 0.01) for treatment 1 (96.78) than 2 (94.34). pH values were lower (P < 0.03) for treatment 2 (3.80) and 4 (3.76), where HCl was added than for 1 (4.06) and 3 (4.16). Ammonia nitrogen values were very low for all treatments. Lactic:acetic acid ratio was large for all treatments (7.55, 14.83, 8.30, 7.63 for treatments 1, 2, 3 and 4, respectively). It is concluded that it is possible to preserve the earthworms E. fetida and L. rubellus by ensiling, adding a source of carbohydrates, such as sorghum or molasses. Not being necessary the addition of acids to have an adequate fermentation.
将赤子爱胜蚓（Eisenia fetida）和红色正蚓（Lumbricus rubellus）与磨碎的高粱和糖蜜按以下比例进行青贮：1）60%蚯蚓、40%高粱；2）60%蚯蚓、40%高粱，用盐酸将pH值调至4.0；3）60%蚯蚓、20%高粱、20%糖蜜；4）60%蚯蚓、20%高粱、20%糖蜜，用盐酸将pH值调至4.0。这些混合物在18摄氏度下发酵15天。对青贮混合物的pH值、近似化学成分分析、可消化蛋白质、真蛋白质、氨氮、乙酸、丙酸、丁酸、乳酸和总能进行了分析。数据采用方差分析和正交对比进行分析。在水分、乙醚提取物、粗纤维和粗蛋白百分比方面未发现差异（P>0.0001）（处理1、2、3和4的水分、乙醚提取物、粗纤维和粗蛋白百分比分别为52.22、2.96、1.15、22.00；51.76、3.48、1.28、20.17；53.89、3.23、0.95、20.63；54.87、2.99、1.03、21.14）。真蛋白质和总能也没有差异（P>0.0001）（处理1、2、3和4的真蛋白质和总能分别为7.57、4.37；6.92、4.41；5.45、4.37；6.38、4.30）。高粱处理组和高粱与糖蜜处理组之间的灰分含量（P<0.0001）和无氮提取物（P<0.02）存在差异（处理1、2、3和4的灰分含量和无氮提取物分别为3.80、70.09；3.60、71.47；6.08、69.11；6.63、68.21）。处理1（96.78）的可消化蛋白质也与处理2（94.34）不同（P<0.01）。添加盐酸的处理2（3.80）和处理4（3.76）的pH值低于处理1（4.06）和处理3（4.16）（P<0.03）。所有处理的氨氮值都非常低。所有处理的乳酸:乙酸比值都很大（处理1、2、3和4的乳酸:乙酸比值分别为7.55、14.83、8.30、7.63）。结论是，通过青贮，添加碳水化合物来源，如高粱或糖蜜，可以保存赤子爱胜蚓和红色正蚓。无需添加酸即可进行充分发酵。
