Yamamoto Y, Maenosono S, Okada H, Miyagawa I, Sofikitis N
Reproductive Physiology and IVF Center, Department of Urology, Tottori University School of Medicine, Yonago, Japan.
Andrologia. 1997 Nov-Dec;29(6):303-10. doi: 10.1111/j.1439-0272.1997.tb00323.x.
The purpose of this study was to compare the morphology/morphometry and fertilizing capacity of human spermatozoa recovered via swim-up method, Percoll density gradient method, and SpermPrep II filtration method. Thirty-three ejaculates were equally divided into 2 aliquots. Aliquot 1 was processed via the direct swim-up method, whereas aliquot 2 was filtered via a SpermPrep II column. The Percoll density gradient method was compared with the SpermPrep II method in a similar protocol using 43 ejaculates. Sperm populations recovered via the SpermPrep II filtration method showed significantly higher hypoosmotic swelling test results, acrosin profiles, and percentage of hyperactivated spermatozoa than sperm fractions recovered by the swim-up method. Furthermore, significant differences were found in most of sperm morphometric parameters between the above sperm populations. However, sperm fractions recovered via the SpermPrep II method did not show significantly different values for these same tests and for most of sperm morphometric parameters compared to the Percoll density gradient method. These results suggest that the SpermPrep II filtration and Percoll density gradient method are equally efficient in isolating sperm subpopulations with better functional parameters than the swimup method.
本研究的目的是比较通过上游法、Percoll密度梯度法和SpermPrep II过滤法回收的人类精子的形态学/形态计量学及受精能力。33份精液样本被平均分成2份。第1份通过直接上游法处理，而第2份通过SpermPrep II柱进行过滤。在一个类似的方案中，使用43份精液样本将Percoll密度梯度法与SpermPrep II法进行了比较。通过SpermPrep II过滤法回收的精子群体在低渗肿胀试验结果、顶体蛋白酶谱以及超活化精子百分比方面显著高于通过上游法回收的精子部分。此外，上述精子群体之间在大多数精子形态计量学参数上存在显著差异。然而，与Percoll密度梯度法相比，通过SpermPrep II法回收的精子部分在这些相同测试以及大多数精子形态计量学参数方面并未显示出显著差异。这些结果表明，SpermPrep II过滤法和Percoll密度梯度法在分离具有比上游法更好功能参数的精子亚群方面同样有效。
