López O, Mata A, Antich M, Bassas L
Laboratory of Andrology, Fundación Puigvert, Barcelona, Spain.
Hum Reprod. 1993 May;8(5):732-6. doi: 10.1093/oxfordjournals.humrep.a138130.
The efficacy of a disposable, prepacked column (PD-10) containing Sephadex G-25, to select motile spermatozoa, was compared with another column for sperm filtration (SpermPrep) and centrifugation through Percoll gradients. Aliquots of washed sperm suspensions were processed by the three techniques. The number of motile cells and the proportion of total spermatozoa selected was similar for all methods. Recovery of spermatozoa showing optimal movement was 145.9 +/- 30% (mean +/- SEM) with PD-10 columns and 131.9 +/- 32% with Percoll, both significantly higher than SpermPrep (71.9 +/- 11%; P < 0.05). The straight line velocity of motile cells was lower in samples processed by SpermPrep (29.3 +/- 2 microns/S) compared to both PD-10 (34.7 +/- 1 micron/s) and Percoll (34.9 +/- 2 microns/s; P = 0.07). When whole semen was processed, total sperm recovery with PD-10 was 61.7 +/- 8% versus 47.7 +/- 7% with Percoll (P < 0.001). Percoll centrifugation improved the percentage of morphologically normal spermatozoa more than PD-10. Similar proportions of motile spermatozoa and cells with optimal motility were obtained by both methods. We conclude that PD-10 filtration columns can be used to prepare semen in the laboratory as a practical alternative to other methods.
将含有葡聚糖凝胶G - 25的一次性预包装柱（PD - 10）用于选择活动精子的效果，与另一种精子过滤柱（SpermPrep）以及通过 Percoll 梯度离心法进行了比较。对洗涤后的精子悬液等分试样采用这三种技术进行处理。所有方法获得的活动细胞数量和所选总精子的比例相似。使用PD - 10柱时，显示最佳运动能力的精子回收率为145.9±30%（平均值±标准误），使用Percoll时为131.9±32%，两者均显著高于SpermPrep（71.9±11%；P < 0.05）。与PD - 10（34.7±1微米/秒）和Percoll（34.9±2微米/秒；P = 0.07）相比，经SpermPrep处理的样本中活动细胞的直线速度较低（29.3±2微米/秒）。当处理全精液时，PD - 10的总精子回收率为61.7±8%，而Percoll为47.7±7%（P < 0.001）。Percoll离心法比PD - 10更能提高形态正常精子的百分比。两种方法获得的活动精子和具有最佳运动能力细胞的比例相似。我们得出结论，PD - 10过滤柱可在实验室用于制备精液，作为其他方法的一种实用替代方法。
