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本文引用的文献

1
The limited strand-separating activity of the UvrAB protein complex and its role in the recognition of DNA damage.UvrAB蛋白复合物有限的链分离活性及其在DNA损伤识别中的作用。
EMBO J. 1997 Feb 17;16(4):889-95. doi: 10.1093/emboj/16.4.889.
2
UvrAB activity at a damaged DNA site: is unpaired DNA present?受损DNA位点处的UvrAB活性：是否存在未配对的DNA？
EMBO J. 1997 Feb 17;16(4):880-8. doi: 10.1093/emboj/16.4.880.
3
Open complex formation around a lesion during nucleotide excision repair provides a structure for cleavage by human XPG protein.在核苷酸切除修复过程中，损伤周围形成的开放复合物为人类XPG蛋白的切割提供了一种结构。
EMBO J. 1997 Feb 3;16(3):625-38. doi: 10.1093/emboj/16.3.625.
4
Formation of DNA repair intermediates and incision by the ATP-dependent UvrB-UvrC endonuclease.DNA修复中间体的形成以及由ATP依赖的UvrB-UvrC核酸内切酶进行的切割。
J Biol Chem. 1997 Feb 21;272(8):4820-7. doi: 10.1074/jbc.272.8.4820.
5
DNA excision repair.DNA切除修复
Annu Rev Biochem. 1996;65:43-81. doi: 10.1146/annurev.bi.65.070196.000355.
6
The C-terminal region of the UvrB protein of Escherichia coli contains an important determinant for UvrC binding to the preincision complex but not the catalytic site for 3'-incision.大肠杆菌UvrB蛋白的C末端区域包含UvrC与切口前复合物结合的重要决定因素，但不包含3'切口的催化位点。
J Biol Chem. 1995 Dec 22;270(51):30508-15. doi: 10.1074/jbc.270.51.30508.
7
Effect of sequence, adduct type, and opposing lesions on the binding and repair of ultraviolet photodamage by DNA photolyase and (A)BC excinuclease.序列、加合物类型及相对损伤对DNA光解酶和（A）BC核酸外切酶结合与修复紫外线光损伤的影响。
J Biol Chem. 1993 May 15;268(14):10694-700.
8
Mechanism of action of the Escherichia coli UvrABC nuclease: clues to the damage recognition problem.大肠杆菌UvrABC核酸酶的作用机制：损伤识别问题的线索
Bioessays. 1993 Jan;15(1):51-9. doi: 10.1002/bies.950150108.
9
Replication of damaged DNA and the molecular mechanism of ultraviolet light mutagenesis.受损DNA的复制及紫外线诱变的分子机制。
Crit Rev Biochem Mol Biol. 1993;28(6):465-513. doi: 10.3109/10409239309085136.
10
The actual incision determines the efficiency of repair of cisplatin-damaged DNA by the Escherichia coli UvrABC endonuclease.实际的切口决定了大肠杆菌UvrABC核酸内切酶修复顺铂损伤DNA的效率。
Biochemistry. 1994 Feb 22;33(7):1804-11. doi: 10.1021/bi00173a025.

UvrABC的一种特异性3'核酸外切酶活性。

A specific 3' exonuclease activity of UvrABC.

作者信息

Gordienko I, Rupp W D

机构信息

Yale University School of Medicine, Department of Therapeutic Radiology, New Haven, CT 06520-8040, USA.

出版信息

EMBO J. 1998 Jan 15;17(2):626-33. doi: 10.1093/emboj/17.2.626.

DOI:10.1093/emboj/17.2.626
PMID:9430653
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1170412/
Abstract

Specific cutting of undamaged DNA by UvrABC nuclease is observed. It occurs seven nucleotides (nt) from the 3' terminus of oligonucleotides annealed to single-stranded M13 DNA circles. Although the location of the UvrABC cut on undamaged DNA is similar to that of the cut on the 5' side of a damaged DNA site during the dual incision reaction, the cut of undamaged DNA is not an intermediate in the dual incision step. On DNA duplexes with a single AAF adduct, the anticipated cut at the eighth phosphodiester bond 5' of the lesion is present, but extra cuts at 7-nt increments are observed at the 15th and 22nd phosphodiester bonds. We suggest that these additional cuts are made by the UvrABC activity observed on undamaged DNA; such activity is referred to as ABC 3' exonuclease and may play a significant role by providing a suitable gap for RecA-mediated recombinational exchanges during repair of interstrand crosslinks and closely opposed lesions. This ABC 3' exonuclease activity depends on higher concentrations of Uvr proteins as compared with dual incision and may be relevant to reactions that occur when UvrA and UvrB are increased during SOS induction.

摘要

观察到UvrABC核酸酶对未受损DNA的特异性切割。这种切割发生在与单链M13 DNA环退火的寡核苷酸3'末端的七个核苷酸（nt）处。尽管未受损DNA上UvrABC切割的位置与双切口反应中受损DNA位点5'侧的切割位置相似，但未受损DNA的切割不是双切口步骤中的中间体。在具有单个AAF加合物的DNA双链体上，在损伤位点5'的第八个磷酸二酯键处存在预期的切割，但在第15个和第22个磷酸二酯键处观察到以7 nt增量的额外切割。我们认为这些额外的切割是由在未受损DNA上观察到的UvrABC活性产生的；这种活性被称为ABC 3'核酸外切酶，在链间交联和紧密相邻损伤的修复过程中，可能通过为RecA介导的重组交换提供合适的缺口而发挥重要作用。与双切口相比，这种ABC 3'核酸外切酶活性依赖于更高浓度的Uvr蛋白，并且可能与SOS诱导期间UvrA和UvrB增加时发生的反应有关。