Vadokas B, Lüdtke F E, Lepsien G, Golenhofen K, Mandrek K
Department of General Surgery, University of Göttingen, Germany.
Neurogastroenterol Motil. 1997 Dec;9(4):265-70. doi: 10.1046/j.1365-2982.1997.d01-59.x.
Mechanical activity was recorded in muscle preparations isolated from the human ileocaecal region. Gastrin-releasing peptide (GRP, 10(-9)-10(-7) mol L-1) produced two types of response in the different muscle layers. Longitudinally cut strips showed a concentration-dependent increase in the rhythmic activity, whereas the circularly orientated layers generally reacted with a small decrease in tone. These effects could not be influenced by blockade of adrenergic or cholinergic receptors or nerve blockade with tetrodotoxin (TTX). Application of pentagastrin did not mimic the action of GRP. These findings suggest a direct action of GRP on smooth muscle via distinct receptors which have already been demonstrated to exist in human gastrointestinal tract. The opposite effects on circular and longitudinal strips might indicate a modulatory role of GRP in the control of ileocolonic transit.
在从人回盲部分离出的肌肉标本中记录机械活动。胃泌素释放肽（GRP，10⁻⁹ - 10⁻⁷ mol/L）在不同肌肉层产生两种类型的反应。纵向切割的条带显示节律性活动呈浓度依赖性增加，而环形排列的层通常表现为张力略有降低。这些效应不受肾上腺素能或胆碱能受体阻断或用河豚毒素（TTX）进行神经阻断的影响。五肽胃泌素的应用不能模拟GRP的作用。这些发现表明GRP通过已证实在人胃肠道中存在的不同受体对平滑肌有直接作用。对环形和纵向条带的相反作用可能表明GRP在回结肠运输控制中具有调节作用。
