通过腹腔渗出细胞在体外恢复白血病病毒抑制的免疫细胞。

Restoration of leukemia virus-suppressed immunocytes in vitro by peritoneal exudate cells.

作者信息

Specter S, Patel N, Friedman H

出版信息

Proc Soc Exp Biol Med. 1976 Jan;151(1):163-7. doi: 10.3181/00379727-151-39166.

Abstract

Depressed antibody responsiveness to sheep erythrocytes in mice infected with Friend leukemia virus continued in vitro when spleen cell cultures from infected animals were cultured in the presence of antigen. Addition of PE cells from normal donor mice to the immunologically depressed splenocyte cultures resulted in a marked restoration of antibody responsiveness. Restoration of the immune response was PE cell dose-dependent; a ratio of 1 PE cell per 10 splenocytes resulted in the largest numbers of PFC's. These results suggest that impaired antibody responsiveness by spleen cell cultures from FLV-infected mice may be due, in part, to effects on antigen-processing macrophages, since restoration of immune responsiveness occurs by PE cell supplements.

摘要

感染了弗瑞德白血病病毒的小鼠，其对绵羊红细胞的抗体反应性降低，当将感染动物的脾细胞培养物在抗原存在的情况下进行体外培养时，这种降低的反应性仍会持续。向免疫抑制的脾细胞培养物中添加来自正常供体小鼠的PE细胞，会导致抗体反应性显著恢复。免疫反应的恢复呈PE细胞剂量依赖性；每10个脾细胞中有1个PE细胞的比例会产生最多数量的空斑形成细胞（PFC）。这些结果表明，来自感染FLV小鼠的脾细胞培养物导致的抗体反应性受损，可能部分归因于对抗抗原处理巨噬细胞的影响，因为通过添加PE细胞可恢复免疫反应性。