Ali A T, al-Swayeh O A, al-Humayyd M S, Mustafa A A, al-Rashed R S, al-Tuwaijiri A S
Department of Medical Pharmacology, College of Medicine, King Saud University, Riyadh, Saudi Arabia.
Eur J Gastroenterol Hepatol. 1997 Nov;9(11):1101-7. doi: 10.1097/00042737-199711000-00014.
It has been proposed that natural honey may contain a 'sucralfate-like' substance. Recent studies have shown that sucralfate affords protection against ischaemia-reperfusion-induced injuries in the rat stomach. Therefore, the effect of honey was studied on ischaemia-reperfusion-induced gastric lesions, intraluminal bleeding, vascular permeability and non-protein sulphhydryls (NP-SH) in the rat stomach.
Rats were subjected to 30 min of gastric ischaemia in the presence of 100 mM HCl and reperfusion period of 60 min. Intraluminal bleeding was assessed macroscopically and the gastric lesions were graded microscopically under an inverted microscope. Vascular permeability was quantified by measuring spectrophotometrically the extravasated Evans blue dye in the stomach. NP-SH levels were measured spectrophotometrically. A luminol-dependent chemiluminescence method was used to assess antioxidant effects of honey in vitro.
There were significantly more gastric lesions, more severe intraluminal bleeding, more leakage of Evans blue and depletion of NP-SH during the reperfusion period as compared to controls. Pre-treatment with honey (0.078-0.625 g/kg, orally) or dimethyl sulphoxide (0.02-0.08 g/kg, intraperitoneally) 30 min before the ischaemia-reperfusion dose-dependently reduced the gastric lesions and intraluminal bleeding and decreased the vascular permeability. Furthermore, honey reversed the ischaemia-reperfusion-induced depletion of NP-SH levels and inhibited the luminol-dependent chemiluminescence induced in a cell-free xanthine-xanthine oxidase system.
These results suggest that gastric protection by honey may be a result of its antioxidant effect. It is suggested that this property of honey may be due to the presence of a 'sucralfate-like' substance.
有人提出天然蜂蜜可能含有一种“硫糖铝样”物质。最近的研究表明，硫糖铝能保护大鼠胃免受缺血再灌注损伤。因此，研究了蜂蜜对大鼠胃缺血再灌注诱导的胃损伤、腔内出血、血管通透性和非蛋白巯基（NP-SH）的影响。
大鼠在100 mM盐酸存在下进行30分钟的胃缺血，再灌注60分钟。宏观评估腔内出血情况，并在倒置显微镜下对胃损伤进行微观分级。通过分光光度法测量胃内渗出的伊文思蓝染料来定量血管通透性。分光光度法测定NP-SH水平。采用鲁米诺依赖的化学发光法体外评估蜂蜜的抗氧化作用。
与对照组相比，再灌注期间胃损伤明显更多、腔内出血更严重、伊文思蓝渗漏更多且NP-SH耗竭。在缺血再灌注前30分钟用蜂蜜（0.078 - 0.625 g/kg，口服）或二甲亚砜（0.02 - 0.08 g/kg，腹腔注射）预处理，剂量依赖性地减少了胃损伤和腔内出血，并降低了血管通透性。此外，蜂蜜逆转了缺血再灌注诱导的NP-SH水平耗竭，并抑制了无细胞黄嘌呤 - 黄嘌呤氧化酶系统中诱导的鲁米诺依赖的化学发光。
这些结果表明蜂蜜对胃的保护作用可能是其抗氧化作用的结果。有人认为蜂蜜的这种特性可能归因于一种“硫糖铝样”物质的存在。
