Sharma S, Brousseau R, Kasatiya S
Ottawa Public Health Laboratory, Ontario, Canada.
J Clin Microbiol. 1998 Jan;36(1):277-80. doi: 10.1128/JCM.36.1.277-280.1998.
Following the isolation of Mycoplasma pneumoniae from urogenital specimens (M. Goulet, R. Dular, J. G. Tully, G. Billows, and S. Kasatiya, J. Clin. Microbiol. 33:2823-2825, 1995), a study was undertaken to confirm the observations by PCR. Specific primers directed to the P1 adhesin gene of M. pneumoniae were used. A total of 300 genital specimens were tested for M. pneumoniae and Mycoplasma genitalium by culture and PCR. Of these, 15 were positive by culture and 17 were positive by PCR for M. pneumoniae. No M. genitalium was detected in any of the specimens by either method. The present study demonstrates that PCR is sensitive and rapid compared to cumbersome culture methods and can be used to detect M. pneumoniae in urogenital specimens in a routine diagnostic laboratory.
自泌尿生殖系统标本中分离出肺炎支原体后(M. 古莱、R. 杜拉尔、J. G. 塔利、G. 比洛斯和S. 卡萨蒂亚,《临床微生物学杂志》33:2823 - 2825,1995年),开展了一项研究,通过聚合酶链反应(PCR)来证实这些观察结果。使用了针对肺炎支原体P1黏附素基因的特异性引物。通过培养和PCR对总共300份生殖系统标本进行了肺炎支原体和生殖支原体检测。其中,15份通过培养呈阳性,17份通过PCR检测肺炎支原体呈阳性。两种方法在任何标本中均未检测到生殖支原体。本研究表明,与繁琐的培养方法相比,PCR检测灵敏且快速,可用于常规诊断实验室检测泌尿生殖系统标本中的肺炎支原体。