Munakata M, Chen H, Nasuhara Y, Sato A, Takahashi T, Sato R, Homma Y, Kawakami Y
First Department of Medicine, School of Medicine, Hokkaido University, Sapporo, Japan.
Respirology. 1996 Mar;1(1):61-7. doi: 10.1111/j.1440-1843.1996.tb00012.x.
Airway inflammation may cause alteration of airway responses in chronic airway diseases, such as bronchial asthma. The objective of this study was to examine whether interleukin-1beta (IL-1beta) [corrected], one of the pro-inflammatory cytokines, has a direct effect on airway functions. The effects of IL-1beta on carbachol, KCl and isoproterenol (ISO) responses of isolated guinea-pig tracheal strips were examined by measuring isometric tension in tissue bath. Responses of tracheal strips with or without epithelium to each agonist were compared before and after incubation with IL-1beta (25 ng or 250 ng/mL). Both 1 h and 5 h incubation of the strips with 250 ng/mL IL-1beta significantly decreased the sensitivity not only to KCl (P < 0.05; P < 0.01, respectively), but also to ISO (both P < 0.05) without affecting maximum contraction or relaxation. Response to carbachol was not affected by IL-1beta. Epithelial denudation abolished the effects of IL-1beta on KCl and ISO responses. Indomethacin (2 micromol/L) [corrected] reversed the effects of IL-1beta both on KCl and on ISO. These results suggest that IL-1 beta decreases the sensitivity of airway strips to KCl and ISO, possibly by stimulating prostaglandin production from the airway epithelium [corrected].
气道炎症可能会导致慢性气道疾病（如支气管哮喘）中气道反应的改变。本研究的目的是检验促炎细胞因子之一的白细胞介素-1β（IL-1β）[校正后]是否对气道功能有直接影响。通过在组织浴中测量等长张力，研究了IL-1β对分离的豚鼠气管条对卡巴胆碱、氯化钾和异丙肾上腺素（ISO）反应的影响。在与IL-1β（25 ng或250 ng/mL）孵育前后，比较了有或无上皮的气管条对每种激动剂的反应。用250 ng/mL IL-1β孵育气管条1小时和5小时，不仅显著降低了对氯化钾的敏感性（分别为P < 0.05；P < 0.01），还降低了对ISO的敏感性（两者均为P < 0.05），且不影响最大收缩或舒张。对卡巴胆碱的反应不受IL-1β影响。上皮剥脱消除了IL-1β对氯化钾和ISO反应的影响。吲哚美辛（2 μmol/L）[校正后]逆转了IL-1β对氯化钾和ISO的影响。这些结果表明，IL-1β可能通过刺激气道上皮产生前列腺素，降低气道条对氯化钾和ISO的敏感性[校正后]。
