Moreno-Manzano V, Rodriguez-Puyol M, Arribas-Gómez I, Rodríguez-Puyol D, Lucio-Cazaña J
Department of Physiology and Pharmacology, University of Alcalá de Henares, Madrid, Spain.
Int J Vitam Nutr Res. 1997;67(6):427-31.
The present work shows that dietary tretinoin (all-trans-retinoic acid) supplementation may be a useful manoeuvre for the prevention of age-related renal changes. 18-month-old male Fischer 344 rats fed during three months with standard chow plus tretinoin (1 mg/kg/day) did not exhibit any adverse effect in terms of bodyweight, urinary volume, renal handling of sodium and both hematological and blood chemistry parameters. Although the diet did not reduce age-related proteinuria nor renal lipid peroxidation, glomerular filtration rate and renal cortex protein content were, respectively, 30% higher and 30% lower than in age-matched control rats. These results suggest that dietary tretinoin supplementation may be a useful manoeuvre to slow the progression of age-related renal changes. Since glomerular H2O2 production increases during renal aging in rats, we studied the effect of tretinoin on the biology of cultured glomerular rat mesangial cells exposed to H2O2. Preincubation with tretinoin abolished cell proliferation or cell death induced, respectively, by low and high concentrations of H2O2. These results suggest that the modulation of the cellular actions of H2O2 may be relevant in the mechanisms through which tretinoin prevents age-related renal changes.
目前的研究表明，膳食补充维甲酸（全反式维甲酸）可能是预防与年龄相关的肾脏变化的一种有效方法。18个月大的雄性Fischer 344大鼠在三个月内喂食标准饲料加维甲酸（1毫克/千克/天），在体重、尿量、肾脏对钠的处理以及血液学和血液化学参数方面均未表现出任何不良反应。尽管该饮食并未降低与年龄相关的蛋白尿和肾脏脂质过氧化，但肾小球滤过率和肾皮质蛋白含量分别比年龄匹配的对照大鼠高30%和低30%。这些结果表明，膳食补充维甲酸可能是减缓与年龄相关的肾脏变化进展的一种有效方法。由于大鼠肾脏衰老过程中肾小球过氧化氢的产生增加，我们研究了维甲酸对暴露于过氧化氢的培养大鼠肾小球系膜细胞生物学的影响。用维甲酸预孵育可分别消除低浓度和高浓度过氧化氢诱导的细胞增殖或细胞死亡。这些结果表明，过氧化氢细胞作用的调节可能与维甲酸预防与年龄相关的肾脏变化的机制有关。
