Ruiz-Torres P, Gonzalez-Rubio M, Lucio-Cazaña F J, Ruiz-Villaespesa A, Rodriguez-Puyol M, Rodriguez-Puyol D
Department of Physiology and Pharmacology, University of Alcalá de Henares, Madrid, Spain.
J Lab Clin Med. 1994 Oct;124(4):489-95.
This work deals with the hypothesis that an increased synthesis of reactive oxygen species (ROS) or platelet-activating factor (PAF) (or both) may be implied in the genesis of age-related glomerulosclerosis. Plasma concentration and urinary excretion of both thiobarbituric acid-reactive substances (TBARS) and PAF were measured in young and old human beings and rats. Moreover, these same parameters as well as H2O2 synthesis and reduced glutathione (GSH) content were measured in isolated glomeruli of young (3 months) and old (18 months) Wistar rats. H2O2 synthesis and GSH content were also measured in cultured rat mesangial cells from young and old animals. Both human beings and rats showed a decreased glomerular filtration rate and an increased urinary protein excretion with respect to young individuals. Isolated glomeruli from old animals showed a higher protein content and a lower number of cell nuclei than those from young rats. No changes were detected in plasma concentration and urinary excretion of TBARS and PAF in either human beings or rats. Glomeruli from 18-month-old rats exhibited a higher content of TBARS and GSH and an increased synthesis of H2O2 and PAF than did those from 3-month-old rats. GSH content and H2O2 synthesis were higher in cultured cells from old rats than in those from young rats. These results point to the possibility that ROS or PAF could mediate some of the changes that characterize age-related glomerulosclerosis.
本研究探讨了一种假说，即活性氧（ROS）或血小板活化因子（PAF）（或两者）合成增加可能与年龄相关性肾小球硬化的发生有关。我们测定了年轻和老年人类及大鼠血浆中硫代巴比妥酸反应性物质（TBARS）和PAF的浓度以及尿排泄量。此外，我们还测定了年轻（3个月）和老年（18个月）Wistar大鼠分离肾小球中的这些相同参数，以及H2O2合成和还原型谷胱甘肽（GSH）含量。我们还测定了年轻和老年动物培养的大鼠系膜细胞中的H2O2合成和GSH含量。与年轻个体相比，人类和大鼠的肾小球滤过率均降低，尿蛋白排泄增加。老年动物分离的肾小球比年轻大鼠的肾小球蛋白质含量更高，细胞核数量更少。在人类或大鼠中，未检测到TBARS和PAF的血浆浓度及尿排泄量有变化。与3个月龄大鼠的肾小球相比，18个月龄大鼠的肾小球TBARS和GSH含量更高，H2O2和PAF合成增加。老年大鼠培养细胞中的GSH含量和H2O2合成高于年轻大鼠。这些结果表明，ROS或PAF可能介导了一些与年龄相关性肾小球硬化相关的变化。
