大鼠主要组织相容性复合体（MHC）连锁的热休克蛋白70-2（Hsp70-2）和热休克蛋白70-3（Hsp70-3）基因5'侧翼区的分析

Analysis of the 5'-flanking regions of the MHC-linked Hsp70-2 and Hsp70-3 genes of the rat.

作者信息

Friedrich H, Walter L, Günther E

机构信息

Division of Immunogenetics, University of Göttingen, Germany.

出版信息

Biochim Biophys Acta. 1998 Jan 7;1395(1):57-61. doi: 10.1016/s0167-4781(97)00154-1.

DOI:10.1016/s0167-4781(97)00154-1

PMID:9434152

Abstract

We have analyzed the genomic interval between the MHC-linked heat shock protein 70 (Hsp70) genes Hsp70-2 and Hsp70-3 in the rat. The distance between the transcription start sites of both genes which are organized in a head-to-head orientation is 604 bp. Unlike Hsp70-2 the 5'untranslated region of Hsp70-3 is interrupted by an intron of about 3.9 kb. The Hsp70-3 promoter lacks a TATA box and heat shock elements, but contains two CRE elements, one Sp1-GC and two PuF binding sites. Analysis of the Hsp70-2 promoter by CAT assays reveals positive and negative regulatory elements extending into the Hsp70-3 gene.

摘要

我们分析了大鼠中与主要组织相容性复合体（MHC）相关的热休克蛋白70（Hsp70）基因Hsp70 - 2和Hsp70 - 3之间的基因组间隔。以头对头方向排列的这两个基因的转录起始位点之间的距离为604碱基对。与Hsp70 - 2不同，Hsp70 - 3的5'非翻译区被一个约3.9千碱基对的内含子中断。Hsp70 - 3启动子缺乏TATA盒和热休克元件，但含有两个环磷腺苷反应元件（CRE）、一个Sp1 - GC和两个多聚嘧啶结合因子（PuF）结合位点。通过氯霉素乙酰转移酶（CAT）分析对Hsp70 - 2启动子的研究揭示了延伸至Hsp70 - 3基因中的正负调控元件。