Ito Y, Ando A, Ando H, Ando J, Saijoh Y, Inoko H, Fujimoto H
Mitsubishi Kasei Institute of Life Sciences, Machida, Tokyo, 194-8511, Japan.
J Biochem. 1998 Aug;124(2):347-53. doi: 10.1093/oxfordjournals.jbchem.a022118.
The Hsc70t gene is a Hsp70 homolog gene expressed constitutively in spermatids in mice. This gene is linked to two heat-inducible Hsp70 genes, Hsp70.1 and Hsp70.3, located in the MHC class III region. The syntenic region of human chromosome 6 contains the HSPA1B, HSPA1A, and HSPA1L genes. Here, we have isolated a HSPA1L cDNA clone from human testicular cells. The HSPA1L gene contained an intron 13 bp upstream of the initiating ATG. A similar genomic structure was found in the Hsc70t gene. The transcription initiation site of the Hsc70t gene was located at ca. 600 bp upstream of the heat-inducible Hsp70.3 gene, linked head-to-head. Sequence alignment of the mouse and human genes revealed that the human HSPA1L and HSPA1A genes were orthologous to the mouse Hsc70t and Hsp70.3 genes, respectively. Conserved sequence stretches observed in the 5' flanking region and the first exon of the spermatid-specific Hsp70 gene may be involved in regulation of the specific gene expression.
Hsc70t基因是一种在小鼠精子细胞中组成性表达的Hsp70同源基因。该基因与位于MHC III类区域的两个热诱导型Hsp70基因Hsp70.1和Hsp70.3相连。人类6号染色体的同线性区域包含HSPA1B、HSPA1A和HSPA1L基因。在此，我们从人类睾丸细胞中分离出一个HSPA1L cDNA克隆。HSPA1L基因在起始ATG上游13 bp处含有一个内含子。在Hsc70t基因中发现了类似的基因组结构。Hsc70t基因的转录起始位点位于与热诱导型Hsp70.3基因头对头相连的约600 bp上游处。小鼠和人类基因的序列比对显示，人类HSPA1L和HSPA1A基因分别与小鼠Hsc70t和Hsp70.3基因是直系同源的。在精子细胞特异性Hsp70基因的5'侧翼区域和第一个外显子中观察到的保守序列片段可能参与了特定基因表达的调控。
