信息素1（MSG1）及其相关蛋白MRG1共享一个转录激活结构域。

MSG1 and its related protein MRG1 share a transcription activating domain.

作者信息

Shioda T, Fenner M H, Isselbacher K J

机构信息

Laboratory of Tumor Biology, MGH Cancer Center, Massachusetts General Hospital-East, Charlestown 02129, USA.

出版信息

Gene. 1997 Dec 19;204(1-2):235-41. doi: 10.1016/s0378-1119(97)00551-9.

DOI:10.1016/s0378-1119(97)00551-9

PMID:9434189

Abstract

MSG1 is a recently described melanocyte-specific nuclear protein whose biochemical function is unknown [Shioda et al. (1996) Proc. Natl. Acad. Sci. USA 93, 12298-12303]. Two human cDNA sequences found in the EST (expressed sequence tag) database were predicted to encode a small peptide (45 aa) that showed 69% identity to the C-terminal sequence of MSG1, suggesting the existence of a novel MSG1-related protein. Based on these EST sequences, we isolated a novel gene, MRG1 (MSG1-Related Gene 1), by the 5'-RACE (rapid amplification of cDNA ends) technique. The MRG1 mRNA transcript is expressed widely and encodes a nuclear protein that share two highly conserved domains, CR1 (14 aa) and CR2 (approx. 50 aa), with MSG1. The CR2 domain is significantly acidic and activates transcription in yeast cells. The full-length MSG1 and MRG1 fused to GAL4 DNA-binding domain activates transcription in mammalian cells, and this is dependent on the presence of the CR2 domain. These results suggest that MRG1 and MSG1 may function as transcription activators.

摘要

MSG1是一种最近被描述的黑素细胞特异性核蛋白，其生化功能尚不清楚[盐田等人（1996年），《美国国家科学院院刊》93，12298 - 12303]。在EST（表达序列标签）数据库中发现的两个人类cDNA序列被预测编码一个小肽（45个氨基酸），该小肽与MSG1的C端序列有69%的同一性，这表明存在一种新的与MSG1相关的蛋白质。基于这些EST序列，我们通过5'-RACE（cDNA末端快速扩增）技术分离出一个新基因，MRG1（MSG1相关基因1）。MRG1 mRNA转录本广泛表达，编码一种核蛋白，该核蛋白与MSG1共享两个高度保守的结构域，CR1（14个氨基酸）和CR2（约50个氨基酸）。CR2结构域具有显著的酸性，并在酵母细胞中激活转录。与GAL4 DNA结合结构域融合的全长MSG1和MRG1在哺乳动物细胞中激活转录，这取决于CR2结构域的存在。这些结果表明MRG1和MSG1可能作为转录激活因子发挥作用。