Xia Erjie, Wang Yinghao, Bhandari Adheesh, Niu Jizhao, Yang Fan, Yao Zhihan, Wang Ouchen
Department of Surgical Oncology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.
Oncol Lett. 2018 Jul;16(1):105-112. doi: 10.3892/ol.2018.8653. Epub 2018 May 7.
Thyroid cancer is the most common malignancy of the endocrine organs. In order to further understand the tumorigenesis and progression of papillary thyroid carcinoma (PTC), the present study performed whole transcriptome sequence analysis. It was found that Cbp/p300-interacting transactivators with glutamic acid [E] and aspartic acid [D]-rich C-terminal domain 1 (CITED1) was a novel potential PTC-associated gene in thyroid cancer. The expression level and clinicopathological features of CITED1 were then assessed in The Cancer Genome Atlas (TCGA) database. The expression of CITED1 was knocked down and the biological function of CITED1 in PTC cell lines was examined. The results showed that upregulated CITED1 was associated with lymph node metastasis (P=0.006) and clinical stage (P=0.003). In order to differentiate PTC tissues and normal tissues, an area under the curve was constructed of a receiver operating characteristic of 91.3% for the TCGA cohort and 85.3% for a validated cohort. The downregulated expression of CITED1 significantly inhibited cell proliferation, colony formation, migration and invasion in the PTC cell lines. The present study demonstrated that CITED1 is important in the tumorigenesis and metastasis of PTC and may be a potential therapeutic target in PTC.
甲状腺癌是内分泌器官最常见的恶性肿瘤。为了进一步了解甲状腺乳头状癌（PTC）的肿瘤发生和进展，本研究进行了全转录组序列分析。研究发现，富含谷氨酸[E]和天冬氨酸[D]的C末端结构域1的Cbp/p300相互作用反式激活因子（CITED1）是甲状腺癌中一种新的潜在PTC相关基因。随后在癌症基因组图谱（TCGA）数据库中评估了CITED1的表达水平和临床病理特征。敲低CITED1的表达，并检测CITED1在PTC细胞系中的生物学功能。结果显示，CITED1表达上调与淋巴结转移（P=0.006）和临床分期（P=0.003）相关。为了区分PTC组织和正常组织，构建了受试者工作特征曲线下面积，TCGA队列的曲线下面积为91.3%，验证队列的曲线下面积为85.3%。CITED1表达下调显著抑制PTC细胞系中的细胞增殖、集落形成、迁移和侵袭。本研究表明，CITED1在PTC的肿瘤发生和转移中起重要作用，可能是PTC的一个潜在治疗靶点。
