Martini C N, Vaena de Avalos S G, Romero D G, San Martín de Viale L, Vila M de C
Departamento de Química Biológica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, Argentina.
Steroids. 1997 Dec;62(12):767-70. doi: 10.1016/s0039-128x(97)00074-3.
In this paper, we studied the effect of heme availability on corticosterone and aldosterone synthesis in rat adrenal. We found that hemin stimulated corticosterone and aldosterone production in adrenal homogenates in a dose-dependent fashion. Hemin administration to rats also provoked an increase in both corticosterone and aldosterone content in adrenal. 3,5-Diethoxycarbonyl-1,4-dihydro-2,4,6-trimethylpyridine (DDC), an inhibitor of liver ferrochelatase activity, was able to inhibit this enzyme in rat adrenal. This resulted in an impairment of heme concentration and consequently adrenal ALA-synthase and porphyrin content were increased. Thus, it was proven that DDC inhibits heme biosynthesis in adrenal as it does in liver. In vivo experiments with rats showed that DDC was able to partially blocked ACTH-mediated corticosterone and aldosterone production while hemin administration was able to partially restore it. These data indicate that heme availability affects steroid biosynthesis in rat adrenal.
在本文中，我们研究了血红素可用性对大鼠肾上腺皮质酮和醛固酮合成的影响。我们发现，氯化血红素以剂量依赖的方式刺激肾上腺匀浆中皮质酮和醛固酮的产生。给大鼠施用氯化血红素也会引起肾上腺中皮质酮和醛固酮含量的增加。3,5-二乙氧基羰基-1,4-二氢-2,4,6-三甲基吡啶（DDC），一种肝脏亚铁螯合酶活性抑制剂，能够在大鼠肾上腺中抑制该酶。这导致血红素浓度受损，因此肾上腺δ-氨基-γ-酮戊酸合酶和卟啉含量增加。因此，已证明DDC如同在肝脏中一样，在肾上腺中抑制血红素生物合成。对大鼠进行的体内实验表明，DDC能够部分阻断促肾上腺皮质激素介导的皮质酮和醛固酮产生，而施用氯化血红素能够部分恢复其产生。这些数据表明，血红素可用性影响大鼠肾上腺中的类固醇生物合成。
