Peters R R, Farias M R, Ribeiro-do-Valle R M
Department of Pharmacology, Universidade Federal de Santa Catarina, Brazil.
Planta Med. 1997 Dec;63(6):525-8. doi: 10.1055/s-2006-957755.
The anti-inflammatory and antinociceptive actions of the CH2Cl2 extract and semipurified fraction (F-III) from roots of Wilbrandia ebracteata Cogn. have been investigated in rats and mice. The CH2Cl2 extract (1-10 mg/kg, i.p.; ID50 5 mg/kg) and (3-30 mg/kg, p.o.; ID50 15 mg/kg) inhibited, in a dose-related manner, carrageenan-induced paw edema in rats. The subfraction (F-III) from CH2Cl2 extract and compounds isolated as cucurbitacin B and E also inhibited carrageenan-induced edema. The CH2Cl2 extract and F-III also exhibited significant analgesic action in acetic acid-induced pain in mice. In the formalin test, the CH2Cl2 extract (0.3-10 mg/kg, i.p.) and (3-30 mg/kg, p.o.) caused inhibition of the neurogenic (first phase) and inflammatory phase (second phase) of formalin-induced pain. However, the CH2Cl2 extract was more effective in relation to the second phase than in inhibition of the formalin-induced edema. These findings suggest that CH2Cl2 extract has potent anti-inflammatory and analgesic action and that F-III and cucurbitacin B and E may account for these actions.
对无苞刺冠菊（Wilbrandia ebracteata Cogn.）根的二氯甲烷提取物和半纯化级分（F-III）的抗炎和抗伤害感受作用进行了大鼠和小鼠实验研究。二氯甲烷提取物（腹腔注射1-10mg/kg；半数抑制剂量为5mg/kg）和（口服3-30mg/kg；半数抑制剂量为15mg/kg）呈剂量依赖性抑制大鼠角叉菜胶诱导的爪肿胀。二氯甲烷提取物的亚级分（F-III）以及分离得到的化合物葫芦素B和E也抑制角叉菜胶诱导的肿胀。二氯甲烷提取物和F-III对小鼠醋酸诱导的疼痛也表现出显著的镇痛作用。在福尔马林试验中，二氯甲烷提取物（腹腔注射0.3-10mg/kg）和（口服3-30mg/kg）可抑制福尔马林诱导疼痛的神经源性（第一阶段）和炎症性阶段（第二阶段）。然而，二氯甲烷提取物对第二阶段的作用比对福尔马林诱导肿胀的抑制作用更有效。这些研究结果表明，二氯甲烷提取物具有强大的抗炎和镇痛作用，F-III以及葫芦素B和E可能是这些作用的原因。
