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接种方案、盐度和pH值对土壤中多环芳烃(PAHs)降解及引入的PAH降解菌存活的影响。

Impact of inoculation protocols, salinity, and pH on the degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of PAH-degrading bacteria introduced into soil.

作者信息

Kästner M, Breuer-Jammali M, Mahro B

机构信息

Department of Biotechnology II, Technical University of Hamburg-Harburg, Germany.

出版信息

Appl Environ Microbiol. 1998 Jan;64(1):359-62. doi: 10.1128/AEM.64.1.359-362.1998.

Abstract

Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of the introduced bacteria were monitored in native and sterilized soil at different pHs. Introduction with mineral medium inhibited PAH degradation by the autochthonous microflora and by the strains tested. After introduction with water (without increase of the pore water salinity), no inhibition of the autochthonous microflora was observed and both strains exhibited PAH degradation.

摘要

通过应用不同的接种方案,研究了多环芳烃(PAHs)在土壤中的降解以及细菌在土壤中的存活情况。向土壤中接种了少动鞘氨醇单胞菌BA 2和菌株BP 9,它们分别能够降解蒽和芘。在不同pH值的天然土壤和灭菌土壤中监测了土壤细菌和引入细菌的菌落形成单位(CFU)。用矿物培养基接种抑制了本地微生物群落和受试菌株对PAHs的降解。用水接种(不增加孔隙水盐度)后,未观察到对本地微生物群落的抑制作用,且两种菌株均表现出PAHs降解。

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