Ping Lifeng, Guo Qian, Chen Xiaoyang, Yuan Xiaoli, Zhang Chunrong, Zhao Hua
Key Laboratory of Recycling and Eco-treatment of Waste Biomass of Zhejiang Province, Zhejiang University of Science and Technology, Hangzhou, 310023, China.
Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China.
3 Biotech. 2017 May;7(1):56. doi: 10.1007/s13205-017-0704-y. Epub 2017 Apr 25.
In the current study, the PL7 strain was isolated from soil and identified as Raoultella planticola based on its physiological characteristics and 16S rDNA sequence. By the 10th day, the PL7 strain degraded 52.0% of the pyrene (PYR) content and 50.8% of the benzo[a]pyrene (BaP) content in 20 mg L PYR and 10 mg L BaP in the liquid matrix. The half-life of PYR and BaP by PL7 degradation was 8.59 and 9.46 days, respectively. At pH 8.0, the degradation rates of PYR and BaP by PL7 were significantly higher at 30 °C than at 20 and 40 °C. The degradation ability of PL7 differed in red soil, paddy soil and fluvo-aquic soil; red soil produced the fastest degradation rates. The half-life of PYR and BaP by PL7 degradation in red soil was 21.7 and 11.9 days, respectively; however, without PL7 the half-life of PYR in red soil was 91.2 days. This study demonstrated the significant potential of the PL7 strain for bioremediation applications in the liquid matrix and soil contaminated by PAHs.
在本研究中，PL7菌株从土壤中分离得到，并根据其生理特征和16S rDNA序列鉴定为植物拉乌尔菌。到第10天，PL7菌株在液体基质中20 mg/L芘（PYR）和10 mg/L苯并[a]芘（BaP）的情况下，降解了52.0%的PYR含量和50.8%的BaP含量。PL7降解PYR和BaP的半衰期分别为8.59天和9.46天。在pH 8.0时，30°C下PL7对PYR和BaP的降解率显著高于20°C和40°C。PL7在红壤、水稻土和潮土中的降解能力不同；红壤的降解速度最快。PL7在红壤中降解PYR和BaP的半衰期分别为21.7天和11.9天；然而，没有PL7时，红壤中PYR的半衰期为91.2天。本研究证明了PL7菌株在多环芳烃污染的液体基质和土壤生物修复应用中的巨大潜力。
