利用小亚基核糖体DNA第一类内含子区分桃褐腐病菌与核果褐腐病菌和果生链核盘菌。
Use of SSU rDNA group-I intron to distinguish Monilinia fructicola from M. laxa and M. fructigena.
作者信息
Fulton C E, Brown A E
机构信息
Department of Applied Plant Science, Queen's University of Belfast, UK.
出版信息
FEMS Microbiol Lett. 1997 Dec 15;157(2):307-12. doi: 10.1111/j.1574-6968.1997.tb12790.x.
Monilinia fructicola, M. laxa and M. fructigena are the causal agents of brown rot of pome and stone fruits. M. fructicola is not present in Europe and is classed as a quarantine pathogen in EU countries. A 418-bp group-I intron has been located in the small subunit (SSU) rDNA gene of M. fructicola which is absent from M. laxa and M. fructigena. PCR primers specific to the 3'-region of the intron together with the SSU rDNA primer NS5 were able to amplify a 444-bp product from M. fructicola and fruit tissue infected with M. fructicola but not from the other two species. This allows for the rapid and sensitive detection of this pathogen in planta.
桃褐腐病菌、果生链核盘菌和核果链核盘菌是梨果和核果褐腐病的病原菌。桃褐腐病菌在欧洲不存在，在欧盟国家被列为检疫性病原菌。在桃褐腐病菌的小亚基（SSU）核糖体DNA基因中定位到一个418 bp的I类内含子，而果生链核盘菌和核果链核盘菌中没有该内含子。针对内含子3'区域的PCR引物与SSU rDNA引物NS5一起，能够从桃褐腐病菌和感染桃褐腐病菌的果实组织中扩增出一个444 bp的产物，但不能从其他两个物种中扩增出该产物。这使得能够在植物中快速、灵敏地检测到这种病原菌。
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