Tsunoda Y, Chang M C
J Reprod Fertil. 1976 Mar;46(2):379-82. doi: 10.1530/jrf.0.0460379.
Female rats and mice were injected with homologous spermatozoa without adjuvant, or with homogenates of ovary or testis+ epididymis in adjuvant. Ovulation was not inhibited and the numbers of eggs released were normal. Fertilization rates were significantly reduced in the mouse by all tissues injected and in the rat by injection of ovarian homogenate only. Fracture of and a weak precipitate on the zona pellucida of the eggs were observed in the mouse but not in the rat after injection of ovarian homogenates. The number of pregnancies and litter sizes were reduced following the first and second mating after treatment in the mouse but not in the rat. When eggs with or without follicular cells were examined in vitro, fertilizability was very low if the donor had been treated with ovarian homogenate, better with testis+epididymis homogenate and best after treatment with sperm suspension. Failure of fertilization appeared to be the major cause of infertility after such treatments.
给雌性大鼠和小鼠注射无佐剂的同源精子，或注射佐剂中的卵巢或睾丸+附睾匀浆。排卵未受抑制，排出的卵子数量正常。在小鼠中，所有注射组织均显著降低受精率，在大鼠中，仅注射卵巢匀浆会降低受精率。注射卵巢匀浆后，在小鼠中观察到卵透明带断裂和弱阳性沉淀，但在大鼠中未观察到。处理后的小鼠在第一次和第二次交配后怀孕数量和窝仔数减少，但大鼠未出现此情况。当体外检查有无卵泡细胞的卵子时，如果供体用卵巢匀浆处理，受精能力非常低，用睾丸+附睾匀浆处理时受精能力较好，用精子悬液处理后受精能力最佳。受精失败似乎是此类处理后不育的主要原因。
