Tung K S, Goldberg E H, Goldberg E
J Reprod Immunol. 1979 Oct;1(3):145-58. doi: 10.1016/0165-0378(79)90015-9.
Female Swiss Webster mice were immunized intraperitoneally with mouse epididymal spermatozoa or with phosphate buffered saline (PBS) and their fertility was compared by (1) incidence and size of litters, (2) number of uterine implantation sites, and (3) incidence and number of fertilized eggs in the oviducts. Statistically significant reduction in fertility was noted following two courses of injections of spermatozoa; 12% of mice injected with spermatozoa had litters compared with 80% of mice injected with PBS. The infertility did not seem to be related to a failure in fertilization since the two groups of mice had a similar incidence and number of fertilized eggs in the oviducts. All female mice were found to have a "natural' anti-acrosomal antibody. Following immunization with spermatozoa, antibodies to "postacrosomal' region, the main piece and the midpiece of the tail, as well as cytotoxic antisperm antibodies, appeared. Anti-LDH-X antibody was not detected. However, correlation was not found between infertility and antisperm antibodies or sperm granulomata that developed in the peritoneal cavities. It is concluded that female mice receiving repeated i.p, injections of mouse spermatozoa become infertile and that the infertility is related to interference with events after fertilization.
将雌性瑞士韦伯斯特小鼠腹腔注射小鼠附睾精子或磷酸盐缓冲盐水（PBS），并通过以下方式比较它们的生育能力：（1）产仔数和窝大小；（2）子宫着床部位数量；（3）输卵管中受精卵的发生率和数量。在注射两次精子后，生育能力出现了统计学上的显著下降；注射精子的小鼠中有12%产仔，而注射PBS的小鼠中有80%产仔。不育似乎与受精失败无关，因为两组小鼠输卵管中受精卵的发生率和数量相似。发现所有雌性小鼠都有“天然”抗顶体抗体。在用精子免疫后，出现了针对“顶体后”区域、尾部主段和中段的抗体，以及细胞毒性抗精子抗体。未检测到抗乳酸脱氢酶-X抗体。然而，在不育与抗精子抗体或腹膜腔中形成的精子肉芽肿之间未发现相关性。得出的结论是，接受反复腹腔注射小鼠精子的雌性小鼠会不育，且不育与受精后事件受到干扰有关。
