Mason H D, Cwyfan-Hughes S, Holly J M, Franks S
Department of Obstetrics and Gynaecology, Imperial College School of Medicine at St. Mary's, Paddington, London, UK.
J Clin Endocrinol Metab. 1998 Jan;83(1):284-7. doi: 10.1210/jcem.83.1.4630.
Several studies have now documented the existence of IGFBPs in follicular fluid and their correlation with the health of the follicle. In particular, increased levels of IGFBP-4 have been reported in androgen-dominant atretic follicles and those from polycystic ovaries. The aim of this study was to elucidate the role of IGFBP-4 in ovarian steroidogenesis. Granulosa cells and theca tissue were incubated with or without LH or FSH in the presence or absence of IGFBP-4 (0.5-50 ng/ml). Inhibition by IGFBP-4 of estradiol production in the presence of testosterone alone was seen in three of four experiments. IGFBP-4 completely inhibited FSH-stimulated estradiol production in three experiments and caused 67% inhibition in a fourth. Similar results were obtained for theca, in which concurrent incubation with IGFBP-4 completely negated the stimulatory effects of LH on androstenedione production. The mechanism by which IGFBP-4 exerts these potent effects and the possibility that this may by IGF-independent are currently being investigated.
现在有几项研究记录了卵泡液中胰岛素样生长因子结合蛋白（IGFBPs）的存在及其与卵泡健康的相关性。特别是，在雄激素占主导的闭锁卵泡和多囊卵巢的卵泡中，已报道胰岛素样生长因子结合蛋白-4（IGFBP-4）水平升高。本研究的目的是阐明IGFBP-4在卵巢类固醇生成中的作用。在有或没有IGFBP-4（0.5 - 50 ng/ml）的情况下，将颗粒细胞和卵泡膜组织与促黄体生成素（LH）或促卵泡生成素（FSH）一起孵育。在四个实验中的三个实验中，观察到在仅存在睾酮的情况下，IGFBP-4对雌二醇生成有抑制作用。在三个实验中，IGFBP-4完全抑制了FSH刺激的雌二醇生成，在第四个实验中导致67%的抑制。卵泡膜也获得了类似的结果，其中与IGFBP-4同时孵育完全消除了LH对雄烯二酮生成的刺激作用。目前正在研究IGFBP-4发挥这些强大作用的机制以及这可能不依赖于胰岛素样生长因子（IGF）的可能性。
