Ozen M, Multani A S, Kuniyasu H, Chung L W, von Eschenbach A C, Pathak S
Department of Cell Biology, University of Texas M.D. Anderson Cancer Center, Houston, USA.
Oncol Res. 1997;9(8):433-8.
The chromosomal constitutions of three murine cell lines that developed in vitro from tumors that grew in nude mice after orthotopic and ectopic injections of three human prostate tumor cell lines were examined by histopathology, conventional G-banding, and with fluorescence in situ hybridization techniques. All three murine cell lines showed unique marker chromosomes involving mouse chromosome 12, with a common break point. Histopathologic evidence from a murine prostate gland into which SP 3031 cells had been injected indicated dysplastic glandular epithelium and carcinomatous areas. These observations further indicate that: (a) human prostate tumors are capable of transforming host organ cells, (b) host cells have specific chromosomal alterations that may be associated with transformation, and (c) the process of host cell transformation can be demonstrated in histological sections. Although cancer cell heterogeneity and drug-resistant phenotypes are caused by additional genetic alterations and clonal evolution of the original tumor, transformation of the host's distant organ cells may also contribute because most therapies are directed only to the original cancer cells.
通过组织病理学、传统G显带以及荧光原位杂交技术，对三种人前列腺肿瘤细胞系经原位和异位注射后在裸鼠体内生长的肿瘤体外培养得到的三种鼠细胞系的染色体组成进行了检查。所有三种鼠细胞系均显示出涉及小鼠12号染色体的独特标记染色体，且有一个共同的断点。向鼠前列腺内注射SP 3031细胞后的组织病理学证据表明存在发育异常的腺上皮和癌区。这些观察结果进一步表明：(a)人前列腺肿瘤能够转化宿主器官细胞；(b)宿主细胞具有可能与转化相关的特定染色体改变；(c)宿主细胞转化过程可在组织学切片中得到证实。尽管癌细胞的异质性和耐药表型是由原始肿瘤的额外基因改变和克隆进化引起的，但宿主远处器官细胞的转化也可能起作用，因为大多数治疗仅针对原始癌细胞。
