Köhler H, Lemser B, Müller G, Saalmüller A
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena, Germany.
Vet Immunol Immunopathol. 1997 Sep 19;58(3-4):277-86. doi: 10.1016/s0165-2427(97)00023-8.
The phenotypic composition of bronchoalveolar lymphocytes (BALL) of healthy untreated pigs and of pigs immunized via aerosol with a live Pasteurella multocida mutant was measured cytofluorometrically in comparison to blood lymphocytes (PBL). Cells were analyzed using monoclonal antibodies against porcine IgM, IgA, IgG and SWC3 in single-colour fluorescence and the following antibody combinations for two-colour fluorescence: CD2/SWC1, SWC1/CD25 and CD4/CD8. In nonimmunized pigs, differences between lymphocyte subsets derived from blood and lung were obvious. The numbers of CD2-SWC1+ T-cells, CD25+ activated lymphocytes and IgG+ cells were significantly higher in the bronchoalveolar compartment. Aerosol immunization with P. multocida caused only insignificant changes in the phenotypic composition of PBL whereas the composition of lung-derived lymphocytes changed markedly. The percentage of CD2-SWC1+ lymphocytes and of CD25+ cells coexpressing the SWC1 antigen in BALL increased up to 10 days after aerosol administration, whereas the percentage of all Ig+ cells decreased. These data reflect an early cellular reaction to an aerogenic immunization with a P. multocida aerosol, characterized by an increase in CD2+ T-cells and a T-cell activation.
通过细胞荧光测定法，对健康未处理猪以及经气溶胶接种多杀性巴氏杆菌活突变株免疫的猪的支气管肺泡淋巴细胞（BALL）的表型组成进行了测定，并与血液淋巴细胞（PBL）进行比较。使用针对猪IgM、IgA、IgG和SWC3的单克隆抗体进行单染色荧光分析细胞，并使用以下抗体组合进行双染色荧光分析：CD2/SWC1、SWC1/CD25和CD4/CD8。在未免疫的猪中，源自血液和肺的淋巴细胞亚群之间存在明显差异。支气管肺泡隔中CD2 - SWC1 + T细胞、CD25 + 活化淋巴细胞和IgG + 细胞的数量显著更高。用多杀性巴氏杆菌进行气溶胶免疫仅导致外周血淋巴细胞（PBL）的表型组成发生不显著变化，而肺源性淋巴细胞的组成则发生了明显变化。在气溶胶给药后长达10天，BALL中CD2 - SWC1 + 淋巴细胞和共表达SWC1抗原的CD25 + 细胞的百分比增加，而所有Ig + 细胞的百分比下降。这些数据反映了对多杀性巴氏杆菌气溶胶进行气雾免疫的早期细胞反应，其特征是CD2 + T细胞增加和T细胞活化。
