Berndt A, Müller G
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Jena Branch, Germany.
Vet Immunol Immunopathol. 1995 Nov;49(1-2):143-59. doi: 10.1016/0165-2427(95)05457-h.
The defence mechanisms of the lung acting against airborne antigens (bacteria) are not yet fully understood. Therefore, we investigated cell-derived immunological processes in porcine pulmonary tissue. The incidence and distribution of leukocyte subpopulations in lungs, lung lymph nodes and tonsils from eight animals intratracheally infected with Pasteurella multocida (P.m.) type A wild strain and from four non-infected control animals were established by immunohistochemistry, using a panel of monoclonal antibodies (mAbs) against specific porcine leukocyte antigens: MHC Class II (MHC II), SWC1, SWC3a, CD2a, CD4a, CD8b. In the test animals, a broad layer of SWC1+ cells and SWC3a+ cells emerged in the subsinusoidal region of lung lymph nodes and in the bronchoalveolar spaces of the lung as early as 4 and 24 h after infection, and in the subsinusoidal regions of the lung lymph nodes only very few cells could be stained with the mAb against the MHC II antigen. We could not detect any MHC Class II+ cells in the bronchoalveolar spaces at this time of the investigation. In the course of the disappearance of the SWC1+ cells and SWC3+ cells, CD2a+, CD4a+ and, in lower numbers, CD8b+ T lymphocytes seemed to concentrate in the perivascular and, partially, the peribronchial regions of the lung 72 h after infection. By means of the double immunostaining method, we could demonstrate an accumulation of CD4a+ cells and CD8b+ cells after infection which lacked the SWC1 antigen, indicating that these cells were activated T lymphocytes. The same cell types (SWC1-/CD4a+ and SWC1-/CD8b+ cells) as well as CD8b-/CD4a+ cells could be observed in the interstitium of the lung 72 h after infection.
肺部针对空气传播抗原（细菌）的防御机制尚未完全明确。因此，我们研究了猪肺组织中细胞衍生的免疫过程。通过免疫组织化学方法，使用一组针对特定猪白细胞抗原的单克隆抗体（mAb）：MHC II类（MHC II）、SWC1、SWC3a、CD2a、CD4a、CD8b，确定了8只经气管内感染A型多杀性巴氏杆菌（P.m.）野生株的动物以及4只未感染对照动物的肺、肺淋巴结和扁桃体中白细胞亚群的发生率和分布。在试验动物中，早在感染后4小时和24小时，肺淋巴结的窦下区域和肺的支气管肺泡间隙就出现了一层广泛的SWC1 +细胞和SWC3a +细胞，而在肺淋巴结的窦下区域，只有极少数细胞能用抗MHC II抗原的mAb染色。在本研究的这个时间点，我们在支气管肺泡间隙中未检测到任何MHC II +细胞。在SWC1 +细胞和SWC3 +细胞消失的过程中，感染后72小时，CD2a +、CD4a +以及数量较少的CD8b + T淋巴细胞似乎集中在肺的血管周围和部分支气管周围区域。通过双重免疫染色法，我们可以证明感染后缺乏SWC1抗原的CD4a +细胞和CD8b +细胞的积累，表明这些细胞是活化的T淋巴细胞。感染后72小时，在肺间质中可以观察到相同的细胞类型（SWC1 - /CD4a +和SWC1 - /CD8b +细胞）以及CD8b - /CD4a +细胞。
