Sumitomo M, Suda S, Shindo K, Fukumura M, Fukushima J, Okuda K, Ito A
Division of Clinical Labolatory, Yokohama City University School of Medicine.
Arerugi. 1997 Nov;46(11):1136-47.
In order to investigate the role of tumor necrosis factor alpha (TNF-alpha) in bronchial asthma or chronic respiratory infection, we measured serum levels of TNF-alpha and serum soluble tumor necrosis factor-receptor I (sTNF-RI) in asthmatic patients (n = 11) and patients (n = 10) with chronic respiratory infection by Pseudomonas aeruginosa. We also measured serum levels of eosinophil cationic protein (ECP) in the asthmatic patients. The serum levels of TNF-alpha in the asthmatic patients, patients with chronic respiratory tract infection and control group were 2.864 +/- 0.719 g/ml, 2.564-1.384 pg/ml and 0.681 +/- 0.453 pg/ml respectively. The levels of the former two groups were higher than those of the control group (p < 0.05). The serum levels of sTNF-RI in the asthmatic patients, the patients with chronic respiratory tract infection, and the control group was 758 +/- 268 pg/ml, 999 +/- 242 pg/ml and 909 +/- 268 pg/ml respectively. The levels of the former two groups did not differ significantly from those of the control group. There were significant correlations between TNF-alpha and sTNF-RI in the control group and in the patients with chronic respiratory tract infection, but there was no significant correlation in the asthmatic patients. In the asthmatic patients. TNF-alpha/s TNF-RI correlated with %best of PEF (r = 0.691, n = 9, p 0.0373). The serum levels of ECP correlated significantly with TNF-alpha, but not with sTNF-RI in the asthmatic patients. It is suggested that TNF-alpha plays a significant role in the pathogenesis of bronchial asthma and chronic respiratory tract infection as a factor causing inflammation and that the increase of TNF-alpha/sTNF-RI reflects the activation of eosinophil functions in an asthmatic attack.
为了研究肿瘤坏死因子α（TNF-α）在支气管哮喘或慢性呼吸道感染中的作用，我们检测了哮喘患者（n = 11）和铜绿假单胞菌引起的慢性呼吸道感染患者（n = 10）血清中TNF-α和血清可溶性肿瘤坏死因子受体I（sTNF-RI）的水平。我们还检测了哮喘患者血清中嗜酸性粒细胞阳离子蛋白（ECP）的水平。哮喘患者、慢性呼吸道感染患者和对照组血清TNF-α水平分别为2.864±0.719 pg/ml、2.564 - 1.384 pg/ml和0.681±0.453 pg/ml。前两组水平高于对照组（p < 0.05）。哮喘患者、慢性呼吸道感染患者和对照组血清sTNF-RI水平分别为758±268 pg/ml、999±242 pg/ml和909±268 pg/ml。前两组水平与对照组无显著差异。对照组和慢性呼吸道感染患者中TNF-α与sTNF-RI之间存在显著相关性，但哮喘患者中无显著相关性。在哮喘患者中，TNF-α/sTNF-RI与%最佳呼气峰流速（PEF）相关（r = 0.691，n = 9，p 0.0373）。哮喘患者血清ECP水平与TNF-α显著相关，但与sTNF-RI无关。提示TNF-α作为一种致炎因子在支气管哮喘和慢性呼吸道感染的发病机制中起重要作用，且TNF-α/sTNF-RI升高反映哮喘发作时嗜酸性粒细胞功能的激活。
