Yamazaki K, Gilg T, Kauert G, von Meyer L, Eisenmenger W
Department of Legal Medicine, St. Marianna University School of Medicine, Kanagawa, Japan.
Nihon Hoigaku Zasshi. 1997 Oct;51(5):380-7.
Fatty acid ethylesters (FAEE) are synthesized from ethanol and fatty acids in the heart and brain. Similarly fatty acid methylesters (FAME) are synthesized from methanol and fatty acids. Whereas methanol reportedly indicates recent or chronic consumption of alcoholic beverages, an elevated serum methanol concentration (SMC) indicates mainly chronic alcohol intake. We compared levels of FAEE and FAME in cardiac and brain tissues with the blood ethanol concentration(BEC) and SMC in 18 alcohol abusers and 29 control subjects without history of alcohol abuse(control) to clarify the relationships between BEC and FAEE levels and that between SMC and FAME levels. We also assessed the possibilities of discriminating the alcohol abusers from the control group and of detecting FAEE and FAME accumulations in cardiac and brain tissues. Levels of FAEE and FAME were determined by gas chromatography (GC) in autopsied cardiac and brain tissues. Heart FAEE (HFAEE) levels correlated with BEC (r = 0.61) and it was possible to distinguish between alcohol abusers and controls using discriminant analysis. HFAEE levels in the alcohol abusing group were elevated even with low BEC. Therefore, HFAEE levels indicate ethanol accumulation in cardiac tissues of alcohol abusers. Brain FAEE (BFAEE) and heart and brain FAME (HFAME and BFAME) levels did not correlated closely with BEC and SMC, respectively. However, there are some possible means of discriminating between the two groups in terms of BEC and BFAEE, and SMC and FAME respectively, using discriminant analysis. Employing this analysis, the rate of misclassification was 17-25.5%. The mean levels of HFAEE, BFAEE, HFAME and BFAME were higher in the alcohol abusing group than in the control group, even when their BEC and SMC were quite low.
脂肪酸乙酯（FAEE）由乙醇和心脏及大脑中的脂肪酸合成。同样，脂肪酸甲酯（FAME）由甲醇和脂肪酸合成。据报道，甲醇表明近期或长期饮用酒精饮料，而血清甲醇浓度（SMC）升高主要表明长期饮酒。我们比较了18名酗酒者和29名无酗酒史的对照受试者（对照组）心脏和脑组织中FAEE和FAME的水平与血液乙醇浓度（BEC）和SMC，以阐明BEC与FAEE水平之间以及SMC与FAME水平之间的关系。我们还评估了区分酗酒者和对照组以及检测心脏和脑组织中FAEE和FAME积累的可能性。通过气相色谱法（GC）测定尸检心脏和脑组织中FAEE和FAME的水平。心脏FAEE（HFAEE）水平与BEC相关（r = 0.61），并且可以使用判别分析区分酗酒者和对照组。即使BEC较低，酗酒组的HFAEE水平也升高。因此，HFAEE水平表明酗酒者心脏组织中乙醇的积累。脑FAEE（BFAEE）以及心脏和脑FAME（HFAME和BFAME）水平分别与BEC和SMC没有密切相关性。然而，使用判别分析分别在BEC和BFAEE以及SMC和FAME方面有一些区分两组的可能方法。采用这种分析，错误分类率为17 - 25.5%。即使酗酒组的BEC和SMC相当低，其HFAEE、BFAEE、HFAME和BFAME的平均水平也高于对照组。
