Tay J I, Rutherford A J, Killick S R, Maguiness S D, Partridge R J, Leese H J
Assisted Conception Unit, Leeds General Infirmary, UK.
Hum Reprod. 1997 Nov;12(11):2451-6. doi: 10.1093/humrep/12.11.2451.
Vascularly perfused Fallopian tubes have been used to study the formation and composition of human tubal fluid and the response to adrenergic agents. An artery serving the tube was cannulated and perfused with Medium 199 supplemented with bovine serum albumin (BSA) and antibiotics. A second cannula was attached to the fimbriated end for native tubal fluid collection. The preparation was viable for up to 2 h. Tubal fluid was only obtained in tubes removed in the proliferative and early secretory phases of the ovarian cycle. Isoproterenol (1 mM) added to the perfusate stimulated fluid production, whereas dibutyryl cyclic AMP (1 mM) reduced fluid formation by 66%. Glucose, pyruvate and lactate concentrations in tubal fluid, measured by microfluorescence assays, were 1.11, 0.14 and 5.4 mM respectively. The concentrations of 17 amino acids in tubal fluid were measured by high performance liquid chromatography following fluorescence derivatization. Arginine (0.19 mM) > alanine (0.11 mM) > glutamate (0.09 mM) were present in highest concentration in all phases of the cycle. All 17 amino acid concentrations in tubal fluid were below those in the vascular perfusate. These data provides the basis for a culture medium whose composition mimics the physiological environment to which early human embryos are exposed.
血管灌注的输卵管已被用于研究人输卵管液的形成、成分以及对肾上腺素能药物的反应。将供应输卵管的动脉插管，并用补充了牛血清白蛋白（BSA）和抗生素的199培养基进行灌注。第二个插管连接到伞端用于收集天然输卵管液。该制备物可存活长达2小时。仅在卵巢周期增殖期和早期分泌期取出的输卵管中获得输卵管液。向灌注液中添加异丙肾上腺素（1 mM）可刺激液体产生，而二丁酰环磷腺苷（1 mM）可使液体形成减少66%。通过微荧光测定法测得的输卵管液中葡萄糖、丙酮酸和乳酸浓度分别为1.11、0.14和5.4 mM。输卵管液中17种氨基酸的浓度通过荧光衍生化后的高效液相色谱法测定。精氨酸（0.19 mM）>丙氨酸（0.11 mM）>谷氨酸（0.09 mM）在周期的所有阶段浓度最高。输卵管液中所有17种氨基酸的浓度均低于血管灌注液中的浓度。这些数据为一种成分模拟早期人类胚胎所处生理环境的培养基提供了基础。
