Rushing P A, Henderson R P, Gibbs J
Department of Psychiatry, Cornell University Medical College, NY, USA.
Peptides. 1998;19(1):175-7. doi: 10.1016/s0196-9781(97)00274-x.
The present study explored the effects of intravenous gastrin-releasing peptide1-27 (GRP) on the postprandial intermeal interval (IMI) when delivered shortly after termination of the first spontaneous nocturnal meal in freely-feeding rats. Undisturbed, ad lib-fed (milk), male rats (n = 11) with chronic inferior vena caval catheters were infused with saline and each of three doses (2.5, 5 and 10 nmol/kg) of GRP in counterbalanced order. Infusions began 5 min after the last lick of the first nocturnal meal and continued for 2 min (60 microliters/min), with delivery of the peptide during the first minute. Infusions and recording of meal data (licks) were fully automated and computer-controlled. Both 5 and 10 nmol/kg of GRP significantly prolonged the IMI by over 50%, but had no effect on the size of the following meal. This is the first demonstration of the prolongation of the IMI by intravenous GRP in undisturbed, freely-feeding rats, and the result suggests that endogenous GRP may play a role in the control of the postprandial intermeal interval.
本研究探讨了在自由进食的大鼠首次夜间自发进食结束后不久静脉注射胃泌素释放肽1 - 27（GRP）对餐后餐间间隔（IMI）的影响。将带有慢性下腔静脉导管的未受干扰、自由进食（牛奶）的雄性大鼠（n = 11）按平衡顺序分别输注生理盐水和三种剂量（2.5、5和10 nmol/kg）的GRP。在首次夜间进食的最后一次舔舐后5分钟开始输注，并持续2分钟（60微升/分钟），肽在第一分钟内输注。输注和进食数据（舔舐次数）的记录完全自动化且由计算机控制。5 nmol/kg和10 nmol/kg的GRP均显著延长IMI超过50%，但对随后一餐的食量没有影响。这是首次证明在未受干扰、自由进食的大鼠中静脉注射GRP可延长IMI，结果表明内源性GRP可能在餐后餐间间隔的控制中发挥作用。
