Bourdoiseau G, Bonnefont C, Hoareau E, Boehringer C, Stolle T, Chabanne L
Département de Pathologie et Clinique des Carnivores Domestiques, Ecole Nationale Vétérinaire de Lyon, France.
Vet Immunol Immunopathol. 1997 Oct 6;59(1-2):21-30. doi: 10.1016/s0165-2427(97)00072-x.
Peripheral blood lymphocyte subpopulations and specific IgG1 and IgG2 antibody levels were studied in 14 dogs naturally infected with Leishmania infantum using flow cytometry and ELISA. Six dogs (Group 1) were asymptomatic, and received no treatment. Samples from this group were collected from D0 to D180. The other eight dogs (Group 2) showed clinical symptoms, and were treated with Glucantime (from D0 to D40), with samples being collected from D0 to D90. Twenty-two healthy dogs were used as a control group (Group 3). The results demonstrated changes in the lymphocyte subsets, as well as a decrease in humoral and cellular immunity, in the infected dogs. Analysis of the B-cell populations of Groups 1 and 2 showed a striking reduction in the number of CD21+ cells. There was also a reduction in the CD5+, CD4+ and CD8+ T-cell populations. Drug therapy was found to partly restore the lost immunity, essentially the cell-mediated immunity. Both IgG1- and IgG2- specific antibodies were detected in sera from the fourteen infected dogs, but the IgG2 subclass appeared to be predominant. A significant decrease in the level of IgG2 antibodies was observed in treated and untreated dogs.
使用流式细胞术和酶联免疫吸附测定法（ELISA），对14只自然感染婴儿利什曼原虫的犬的外周血淋巴细胞亚群以及特异性IgG1和IgG2抗体水平进行了研究。6只犬（第1组）无症状，未接受治疗。该组样本从第0天采集至第180天。另外8只犬（第2组）出现临床症状，用葡糖胺（从第0天至第40天）进行治疗，样本从第0天采集至第90天。22只健康犬用作对照组（第3组）。结果表明，受感染犬的淋巴细胞亚群发生了变化，体液免疫和细胞免疫也有所下降。对第1组和第2组B细胞群体的分析显示，CD21+细胞数量显著减少。CD5+、CD4+和CD8+ T细胞群体也有所减少。发现药物治疗可部分恢复丧失的免疫力，主要是细胞介导的免疫力。在14只受感染犬的血清中均检测到了IgG1和IgG2特异性抗体，但IgG2亚类似乎占主导地位。在接受治疗和未接受治疗的犬中，均观察到IgG2抗体水平显著下降。
