Laboratório de Imunopatologia, Núcleo de Pesquisas em Ciências Biológicas/NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil; Laboratório de Epidemiologia de Doenças Infecciosas e Parasitárias, Departamento de Parasitologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil.
Laboratório de Parasitologia Básica, Departamento de Ciências Biológicas, Universidade Federal de Alfenas, Alfenas, Minas Gerais, Brazil.
Vet J. 2011 Nov;190(2):e43-e47. doi: 10.1016/j.tvjl.2011.04.005. Epub 2011 May 18.
Molecular analysis, serology and immunophenotyping for T lymphocytes and their subsets, B lymphocytes and monocytes were performed on dogs naturally infected with Leishmania infantum. Animals were categorised as asymptomatic dogs I (AD-I), with negative serology and positive molecular results, and asymptomatic dogs II (AD-II), with positive serology and positive molecular results, and these were compared to symptomatic dogs (SD) and control dogs (CD). AD-I exhibited immunophenotypic features similar to those of CD, including isotype profiles and concentrations of monocytes. Similar biomarkers were found in AD-II and SD, such as, higher levels of immunoglobulins IgG, IgG2, IgM and IgA and higher concentrations of eosinophils. High frequencies of T lymphocytes and CD4(+) T cells were observed in both AD-I and AD-II compared to SD, whereas CD8(+) T cells were higher only in AD-II compared with SD. Analysis of B lymphocytes revealed an increased frequency of this cell type only in AD-II animals compared with SD. Asymptomatic dogs appear to have a dichotomous infection spectrum that can influence the humoral and cellular immunological status during canine visceral leishmaniasis.
对自然感染利什曼原虫的犬进行了 T 淋巴细胞及其亚群、B 淋巴细胞和单核细胞的分子分析、血清学和免疫表型分析。将动物分为无症状犬 I（AD-I），其血清学阴性且分子结果阳性，和无症状犬 II（AD-II），其血清学阳性且分子结果阳性，并将其与有症状犬（SD）和对照犬（CD）进行比较。AD-I 表现出与 CD 相似的免疫表型特征，包括同型抗体谱和单核细胞浓度。在 AD-II 和 SD 中也发现了类似的生物标志物，如更高水平的免疫球蛋白 IgG、IgG2、IgM 和 IgA 以及更高浓度的嗜酸性粒细胞。与 SD 相比，AD-I 和 AD-II 中的 T 淋巴细胞和 CD4(+) T 细胞频率更高，而 CD8(+) T 细胞仅在 AD-II 中高于 SD。对 B 淋巴细胞的分析显示，与 SD 相比，仅在 AD-II 动物中这种细胞类型的频率增加。无症状犬似乎具有二分感染谱，这可能会影响犬内脏利什曼病期间的体液和细胞免疫状态。
