Kanistanon D, Neelamek M, Dharakul T, Songsivilai S
Department of Immunology, Faculty of Medicine, Siriraj Hospital, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1997 Sep;15(3):133-40.
The immunoreactivity profiles of plasma samples obtained from patients infected with different hepatitis C virus (HCV) genotypes were studied using immunoblot assay containing multiple HCV antigens. The immunoblot assay was found to be positive in 81.5% of 195 blood donors who had anti-HCV antibodies as detected by second generation enzyme immunoassays. The samples reacted preferentially with the viral core, NS3-1 and NS5 antigens, and these reactivities were not influenced by HCV genotype. However, the reactivities with NS3-2 and NS4 antigens varied depending on HCV genotypes. The samples from patients infected with HCV genotype 1 reacted well with NS3-2 and NS4 antigens whereas those with other genotypes did not. In addition, samples with the unclassified HCV genotype reacted poorly with all antigens, except NS3-1. This study demonstrates the importance of the core, NS3-1 and NS5 antigens in the detection of antibodies against HCV, especially in areas where more than one genotypes of HCV are present. It also demonstrates that there is a need for further improvement of the currently used assays as new HCV genotypes are recently discovered.
使用包含多种丙型肝炎病毒（HCV）抗原的免疫印迹分析法，研究了感染不同HCV基因型的患者血浆样本的免疫反应性谱。在195名通过第二代酶免疫法检测出抗HCV抗体呈阳性的献血者中，免疫印迹分析法检测结果为阳性的比例达81.5%。这些样本优先与病毒核心、NS3-1和NS5抗原发生反应，且这些反应不受HCV基因型的影响。然而，与NS3-2和NS4抗原的反应性因HCV基因型而异。感染HCV 1型的患者样本与NS3-2和NS4抗原反应良好，而感染其他基因型的患者样本则不然。此外，未分类HCV基因型的样本除了与NS3-1抗原外，与所有抗原的反应都较弱。这项研究证明了核心、NS3-1和NS5抗原在检测抗HCV抗体中的重要性，尤其是在存在多种HCV基因型的地区。研究还表明，随着最近发现新的HCV基因型，目前使用的检测方法需要进一步改进。
