Vithayasai V, Sirisanthana T, Sakonwasun C, Suvanpiyasiri C
Department of Microbiology, Faculty of Medicine, Chiang Mai University, Thailand.
Asian Pac J Allergy Immunol. 1997 Sep;15(3):141-6.
Flow cytometer (FACScan) was used to determine the range of T lymphocyte subpopulations in normal Thai blood donors at Maharaj Nakorn Chiang Mai Hospital, Chiang Mai. Reference population consisted of 150 healthy HIV seronegative blood donors. T lymphocyte subsets were analysed using two-color immunophenotyping of peripheral blood lymphocytes with a lysed whole blood technique and enumerated. The study showed that the normal values for CD3+ lymphocytes (percent), CD4+ lymphocytes (percent), CD8+ lymphocytes (percent), CD4/CD8 ratio, absolute CD3+ lymphocyte count, absolute CD4+ lymphocyte count and absolute CD8+ lymphocyte count were 64 +/- 8.8, 36.1 +/- 6.4, 25.7 +/- 7.3, 1.5 +/- 0.6, 1,630 +/- 600 cells/microl, 910 +/- 300 cells/microl and 670 +/- 350 cells/microl, respectively. We found that the values of CD3, CD4 and CD4/CD8 ratio were significantly lower than those in the Caucasians but those of CD8 was not significantly different. This observations have important clinical implication for the use of T lymphocyte subsets measurement, especially in the management of HIV infection in Thais. These normal ranges can be used as a reference for the decisions in clinical practice.
在清迈的玛哈拉吉·纳空清迈医院，使用流式细胞仪（FACScan）测定泰国正常献血者的T淋巴细胞亚群范围。参考人群包括150名健康的HIV血清阴性献血者。采用全血溶血技术对外周血淋巴细胞进行双色免疫表型分析，对T淋巴细胞亚群进行分析并计数。研究表明，CD3⁺淋巴细胞（百分比）、CD4⁺淋巴细胞（百分比）、CD8⁺淋巴细胞（百分比）、CD4/CD8比值、绝对CD3⁺淋巴细胞计数、绝对CD4⁺淋巴细胞计数和绝对CD8⁺淋巴细胞计数的正常值分别为64±8.8、36.1±6.4、25.7±7.3、1.5±0.6、1630±600个细胞/微升、910±300个细胞/微升和670±350个细胞/微升。我们发现，CD3、CD4和CD4/CD8比值的值显著低于白种人，但CD8的值无显著差异。这些观察结果对于T淋巴细胞亚群测量的应用具有重要的临床意义，尤其是在泰国人HIV感染的管理中。这些正常范围可作为临床实践中决策的参考。
