Viyanant V, Krailas D, Sobhon P, Upatham E S, Kusamran T, Chompoochan T, Thammasart S, Prasittirat P
Department of Biology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Asian Pac J Allergy Immunol. 1997 Sep;15(3):153-9.
A monoclonal antibody (MoAb) 1C12 that reacts with a 66 kDa surface tegumental (ST) antigen of adult worms of Fasciola gigantica was used to detect circulating antigen in sera of experimentally and naturally infected cattle. A combination of rabbit anti ST-antigens and MoAb 1C12 were used to capture and detect the circulating antigen in sandwich ELISA. The dilutions of 1:1,000 of rabbit anti ST-antigens and 1:100 for MoAb 1C12 were used to reduce cross-reactivity with other trematodes' antigens. The circulating antigen of F. gigantica was demonstrated in sera of all experimentally infected animals as early as the first week after the infection, and it remained detectable until the experiment was terminated at week 32 after the infection. Of the 97 serum samples from naturally infected cattle, the sensitivity of 86.6% was observed when the cut-off point was calculated from 32 serum specimens from uninfected control calves. The sensitivity increased to 100% when the commercial fetal calf and trematode-free baby calves sera were used for calculation of the control cut-off point. Based on these results, the combination of rabbit anti ST-antigens and MoAb 1C12 sandwich ELISA appeared to be sensitive, specific, and applicable in the immunodiagnosis of fasciolosis in cattle for epidemiological study and monitoring of chemotherapeutic efficacy.
一种与巨片形吸虫成虫的66 kDa表面皮层（ST）抗原发生反应的单克隆抗体（MoAb）1C12，被用于检测实验感染和自然感染牛血清中的循环抗原。在夹心ELISA中，兔抗ST抗原和MoAb 1C12联合用于捕获和检测循环抗原。使用1:1000稀释的兔抗ST抗原和1:100稀释的MoAb 1C12来减少与其他吸虫抗原的交叉反应。早在感染后的第一周，在所有实验感染动物的血清中就检测到了巨片形吸虫的循环抗原，并且在感染后第32周实验结束前一直可检测到。在97份来自自然感染牛的血清样本中，当根据32份未感染对照小牛的血清标本计算临界值时，观察到的敏感性为86.6%。当使用商业胎牛血清和无吸虫的幼牛血清计算对照临界值时，敏感性提高到了100%。基于这些结果，兔抗ST抗原和MoAb 1C12夹心ELISA联合检测似乎具有敏感性、特异性，适用于牛片形吸虫病的免疫诊断，用于流行病学研究和化疗疗效监测。
