Fagbemi B O, Obarisiagbon I O, Mbuh J V
Department of Veterinary Microbiology and Parasitology, University of Ibadan, Nigeria.
Vet Parasitol. 1995 Jun;58(3):235-46. doi: 10.1016/0304-4017(94)00718-r.
Antibodies against a specific 88-kDa antigen of Fasciola gigantica were used for the detection of circulating antigen in the sera of cattle with experimental and natural infections of F. gigantica by a double antibody ELISA. Circulating antigen was detectable as early as the second and third weeks after infection and positive absorbance values were obtained for the entire duration of infection. Absorbance values decreased below the cutoff point 3 weeks after chemotherapy with oxyclozanide. This immunoassay also greatly enhanced the specificity of immunodiagnosis of fasciolosis in naturally infected cattle. The test system has excellent potential for the accurate diagnosis of ruminant fasciolosis.
用针对巨片形吸虫一种特定88 kDa抗原的抗体，通过双抗体ELISA检测实验性和自然感染巨片形吸虫的牛血清中的循环抗原。感染后第二周和第三周即可检测到循环抗原，并且在整个感染期间均获得了阳性吸光度值。用羟氯扎胺化疗3周后，吸光度值降至临界值以下。该免疫测定法还大大提高了自然感染牛片形吸虫病免疫诊断的特异性。该检测系统在准确诊断反刍动物片形吸虫病方面具有极好的潜力。
