Ogata S, Okumura K, Taguchi H
Laboratory of Biological Chemistry, Faculty of Bioresources, Mie University, Japan.
Biosci Biotechnol Biochem. 1997 Dec;61(12):2116-8. doi: 10.1271/bbb.61.2116.
We have investigated the effects of niacin on NAD levels and on DNA repair in human lymphocytes. When lymphocytes were incubated in culture medium with various concentrations of niacin, incubation of lymphocytes with nicotinic acid at 5 microM or nicotinamide at 10 mM caused a 2-3 fold increase in NAD content. Under these conditions lymphocytes were treated with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). Interestingly, the rejoining of DNA strand breaks was promoted by nicotinic acid but nicotinamide inhibited the rejoining.
我们研究了烟酸对人淋巴细胞中烟酰胺腺嘌呤二核苷酸（NAD）水平及DNA修复的影响。当淋巴细胞在含有不同浓度烟酸的培养基中孵育时，用5微摩尔的烟酸或10毫摩尔的烟酰胺孵育淋巴细胞会使NAD含量增加2至3倍。在这些条件下，用N-甲基-N'-硝基-N-亚硝基胍（MNNG）处理淋巴细胞。有趣的是，烟酸促进了DNA链断裂的重新连接，而烟酰胺则抑制了这种重新连接。
