Basu A, Mukhopadhyay A K, Chakrabarti A K, Niyogi S K, Saha M R, Yamasaki S, Takeda Y, Nair G B
Department of Microbiology, National Institute of Cholera & Enteric Diseases, Calcutta.
Indian J Med Res. 1997 Dec;106:491-3.
A total of 196 Vibrio cholerae O1 strains isolated between 1970 and 1996 were biotyped by multiplex PCR, susceptibility to polymyxin B and sensitivity to biotype specific phages. We modified the multiplex PCR by increasing the primer concentration of tcpA to improve the results. Comparison of the results of modified multiplex PCR and sensitivity to biotype specific phages and to polymyxin B showed that multiplex PCR was as efficient as phage typing for biotyping of V. cholerae O1. All the strains of V. cholerae O1 could be accurately distinguished based on polymyxin B sensitivity. Thus our results show that susceptibility of strains of V. cholerae O1 to polymyxin B is the easiest method to biotype V. cholerae O1 and is feasible in most laboratories when compared with multiplex PCR and sensitivity to biotype specific phages.
1970年至1996年间分离出的196株霍乱弧菌O1菌株，通过多重聚合酶链反应（PCR）、对多粘菌素B的敏感性以及对生物型特异性噬菌体的敏感性进行生物分型。我们通过增加tcpA引物浓度对多重PCR进行了改进，以提高结果的准确性。比较改进后的多重PCR结果与对生物型特异性噬菌体及多粘菌素B的敏感性，结果表明，多重PCR在霍乱弧菌O1生物分型方面与噬菌体分型一样有效。基于多粘菌素B敏感性，所有霍乱弧菌O1菌株均可被准确区分。因此，我们的结果表明，霍乱弧菌O1菌株对多粘菌素B的敏感性是霍乱弧菌O1生物分型的最简单方法，与多重PCR和对生物型特异性噬菌体的敏感性相比，在大多数实验室中都是可行的。
