Kasai H
Department of Environmental Oncology, Institute of Industrial Ecological Sciences, University of Occupational and Environmental Health, Kitakyushu, Japan.
Mutat Res. 1997 Dec;387(3):147-63. doi: 10.1016/s1383-5742(97)00035-5.
8-hydroxy-2'-deoxyguanosine (8-OH-dG) was first reported in 1984 as a major form of oxidative DNA damage product by heated sugar, Fenton-type reagents and X-irradiation in vitro. 8-OH-dG has been detected in cellular DNA using an HPLC-ECD method in many laboratories. Analyses of 8-OH-dG in animal organ DNA after the administration of oxygen radical-forming chemicals will be useful for assessments of their carcinogenic risk. Its analysis in human leucocyte DNA and in urine is a new approach to the assessment of an individual's cancer risk due to oxidative stress. The increase of the 8-OH-dG level in the cellular DNA, detected by HPLC-ECD method, was supported by its immunochemical detection and its enhanced repair activity. The validity of the general use of 8-OH-dG as a marker of cellular oxidative stress is discussed.
8-羟基-2'-脱氧鸟苷（8-OH-dG）于1984年首次被报道，是体外加热糖类、芬顿型试剂和X射线照射导致的氧化性DNA损伤产物的主要形式。许多实验室已使用高效液相色谱-电化学检测法（HPLC-ECD）在细胞DNA中检测到8-OH-dG。对给予产生活性氧化学物质后动物器官DNA中的8-OH-dG进行分析，将有助于评估其致癌风险。对人白细胞DNA和尿液中的8-OH-dG进行分析，是评估个体因氧化应激导致癌症风险的一种新方法。通过高效液相色谱-电化学检测法检测到的细胞DNA中8-OH-dG水平的升高，得到了其免疫化学检测及其增强的修复活性的支持。本文讨论了将8-OH-dG普遍用作细胞氧化应激标志物的有效性。
