Hiramatsu N, Shimizu M, Fukada H, Kitamura M, Ura K, Fuda H, Hara A
Department of Biology and Aquaculture, Faculty of Fisheries, Hokkaido University, Japan.
Comp Biochem Physiol C Pharmacol Toxicol Endocrinol. 1997 Oct;118(2):149-57. doi: 10.1016/s0742-8413(97)00084-4.
A specific and sensitive enzyme-linked immunosorbent assay (ELISA) and a single radial immunodiffusion (SRID) were developed for measurement of serum vitellogenin (Vg) levels in Sakhalin taimen (Hucho perryi). Regarding specificity for serum Vg, an antiserum raised against lipovitellin of taimen (a-Lv) was adequate for both assays. ELISA and SRID could detect Vg in serum at concentrations as low as 10 ng/ml and 25 micrograms/ml, respectively. In estrogen administration experiments, the level of serum Vg began clearly increasing within 12 to 24 hr after injection of immature females with estradiol-17 beta (E2). The appearance and levels of Vg in males treated with E2 were delayed and smaller, respectively, than for females. Vg levels varied throughout natural vitellogenesis from 0-4 micrograms/ml (3 years old) to approximately 30 mg/ml (5-6 years old). We observed an early transitory peak of serum Vg levels (primary reaction) at the time of early vitellogenesis and chronic high Vg levels (for 6-7 months) in winter period before ovulation. Changes of serum E2 levels were correlated with Vg levels. However, E2 levels decreased a month earlier than Vg levels near ovulation. It appears that the duration of vitellogenesis in taimen is considerably longer than that in other salmonids, lasting more than 2 years.
开发了一种特异性和敏感性均较高的酶联免疫吸附测定法（ELISA）和单向放射免疫扩散法（SRID），用于测定萨哈林哲罗鱼（Hucho perryi）血清中的卵黄蛋白原（Vg）水平。关于血清Vg的特异性，针对哲罗鱼脂卵黄蛋白产生的抗血清（α-Lv）适用于这两种测定法。ELISA和SRID分别能够检测到浓度低至10 ng/ml和25 μg/ml的血清Vg。在雌激素给药实验中，向未成熟雌性注射17β-雌二醇（E2）后12至24小时内，血清Vg水平开始明显升高。用E2处理的雄性中Vg的出现和水平分别比雌性延迟且更低。在整个自然卵黄发生过程中，Vg水平从0-4 μg/ml（3岁）变化到约30 mg/ml（5-6岁）。我们观察到在早期卵黄发生时血清Vg水平出现早期短暂峰值（初级反应），以及在排卵前的冬季出现慢性高Vg水平（持续6-7个月）。血清E2水平的变化与Vg水平相关。然而，在排卵附近，E2水平比Vg水平提前一个月下降。看来哲罗鱼的卵黄发生持续时间比其他鲑科鱼类长得多，超过2年。
