来自荚膜红细菌的ATP合酶atpHAGDC（F1）操纵子。
The ATP synthase atpHAGDC (F1) operon from Rhodobacter capsulatus.
作者信息
Borghese R, Crimi M, Fava L, Melandri B A
机构信息
Department of Biology, University of Bologna, Italy.
出版信息
J Bacteriol. 1998 Jan;180(2):416-21. doi: 10.1128/JB.180.2.416-421.1998.
Abstract
The atpHAGDC operon of Rhodobacter capsulatus, containing the five genes coding for the F1 sector of the ATP synthase, has been cloned and sequenced. The promoter region has been defined by primer extension analysis. It was not possible to obtain viable cells carrying atp deletions in the R. capsulatus chromosome, indicating that genes coding for ATP synthase are essential, at least under the growth conditions tested. We were able to circumvent this problem by combining gene transfer agent transduction with conjugation. This method represents an easy way to construct strains carrying mutations in indispensable genes.
摘要
荚膜红细菌的atpHAGDC操纵子包含编码ATP合酶F1部分的五个基因，已被克隆和测序。启动子区域已通过引物延伸分析确定。在荚膜红细菌染色体中无法获得携带atp缺失的活细胞，这表明编码ATP合酶的基因是必不可少的，至少在所测试的生长条件下是这样。我们能够通过将基因转移因子转导与接合相结合来规避这个问题。这种方法是构建携带必需基因突变的菌株的一种简便方法。
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