幽门螺杆菌clpB突变体的构建与鉴定及其基因在应激反应中的作用
Construction and characterization of a Helicobacter pylori clpB mutant and role of the gene in the stress response.
作者信息
Allan E, Mullany P, Tabaqchali S
机构信息
Department of Medical Microbiology, St. Bartholomew's and the Royal London School of Medicine and Dentistry, West Smithfield, United Kingdom.
出版信息
J Bacteriol. 1998 Jan;180(2):426-9. doi: 10.1128/JB.180.2.426-429.1998.
Abstract
Antiserum raised against whole Helicobacter pylori cells identified a novel 94-kDa antigen. The nucleotide sequence of the gene encoding the 94-kDa antigen was determined, and analysis of the deduced amino acid sequence revealed structural features typical of the ClpB ATPase family of stress response proteins. An isogenic H. pylori clpB mutant showed increased sensitivity to high-temperature stress, indicating that the clpB gene product functions as a stress response protein in H. pylori.
摘要
针对幽门螺杆菌全菌产生的抗血清鉴定出一种新的94 kDa抗原。测定了编码该94 kDa抗原的基因的核苷酸序列，对推导的氨基酸序列的分析揭示了应激反应蛋白的ClpB ATP酶家族典型的结构特征。一株同源的幽门螺杆菌clpB突变体对高温应激的敏感性增加，表明clpB基因产物在幽门螺杆菌中作为一种应激反应蛋白发挥作用。
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